Synthesis of Nonlinear Optical Dyes for Neurophysiology

用于神经生理学的非线性光学染料的合成

• 批准号: 163727489
• 负责人: Dr. Jan Fleischhauer
• 金额: --
• 依托单位: Chemistry Research Laboratory
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/163727489?language=en
• 关键词: Synthesis; Nonlinear; Optical; Dyes; Neurophysiology

Understanding how the brain works is one of the great unsolved scientific challenges. In order to learn how neuronal networks process information, we need a way of mapping the voltage changes in neurons, with high sensitivity, high spatial resolution and high temporal resolution. Besides the well established routes for measuring changes in cell membrane potentials, such as microelectrodes, Ca2+ -indicators and excited fluorescence spectroscopy, the Second Harmonic Generation (SHG) – microscopy proves to be a promising non-invasive, high sensitive tool. The aim of this research project is to develop suitable SHG-dyes, which match the required properties so that they exhibit a high sensitivity to an external electrical field. Based on the lead structure of a SHG-active porphyrin, which was recently found as promising candidate for SHG-microscopy and can be described as “Push-π-Pull“- chromophor, this goal will be achieved by varying several structural motives (donor, π, acceptor) in the dye.

了解大脑是如何工作的是尚未解决的重大科学挑战之一。为了了解神经元网络如何处理信息，我们需要一种方法来映射神经元中的电压变化，具有高灵敏度，高空间分辨率和高时间分辨率。除了用于测量细胞膜电位变化的成熟途径，如微电极，Ca 2+指示剂和激发荧光光谱，二次谐波发生（SHG）显微镜被证明是一种有前途的非侵入性，高灵敏度的工具。该研究项目的目的是开发合适的SHG染料，这些染料符合所需的特性，因此它们对外部电场具有高灵敏度。基于SHG活性卟啉的先导结构，其最近被发现是SHG显微术的有希望的候选者，并且可以被描述为“推-π-拉”-发色团，该目标将通过改变染料中的几种结构基序（供体、π、受体）来实现。