The role of African trypanosomal trans-sialidases in the transmission of Trypanosoma congolense between the fly vector and life stock host suffering Nagana disease

非洲锥虫唾液酸转移酶在果蝇媒介和患有纳加那病的牲畜宿主之间传播刚果锥虫中的作用

• 批准号: 164877587
• 负责人: Professor Dr. Sörge Kelm
• 金额: --
• 依托单位: Arbeitsgruppe Glycobiochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/164877587?language=en
• 关键词: role; African; trypanosomal; trans; sialidases

African trypanosomiasis, called sleeping sickness in humans and Nagana in cattle, is a disease that is resurgent in Africa. The aim of this project is to test the hypothesis that trans-sialidase (TS) and TS-like proteins play a role in the infection cycle of T. congolense and related trypanosomes, in particular in the establishment of infection in the fly vector and the subsequent trans - mission to mammalian hosts. In close collaboration between the German and Nigerian partners genetic and biochemical approaches will be used to investigate the expression patterns of TS and TS-like genes of T. congolense and T. brucei during the stages in the trypanosome life cycle, study the expression pattern heterogeneity in the field, characterise the enzymatic activity and specificity of the gene products and probe the effects of inhibiting the trypanosomal TS activity in flies and mammalian hosts.

非洲锥虫病，在人类中被称为昏睡病，在牛中被称为纳加纳病，是一种在非洲卷土重来的疾病。该项目的目的是检验转唾液酸酶（TS）和 TS 样蛋白在刚果锥虫和相关锥虫的感染周期中发挥作用的假设，特别是在果蝇媒介中建立感染以及随后传播到哺乳动物宿主中。德国和尼日利亚合作伙伴密切合作，将利用遗传和生化方法研究刚果锥虫和布氏锥虫的 TS 和 TS 样基因在锥虫生命周期各阶段的表达模式，研究现场表达模式的异质性，表征基因产物的酶活性和特异性，并探讨抑制锥虫 TS 活性的效果。 苍蝇和哺乳动物宿主。