JSPS Postdoctoral Fellowship: Functional Labeling of Olfactory Receptor Neurons

JSPS 博士后奖学金：嗅觉感受器神经元的功能标记

• 批准号: 0343958
• 负责人: Jason Sandahl
• 金额: $ 0.24万
• 依托单位: Sandahl Jason F
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-15 至 2005-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0343958&HistoricalAwards=false
• 关键词: JSPS; Postdoctoral; Fellowship; Functional; Labeling

0343958SandahlThis award supports a one-year Japan Society for the Promotion of Science (JSPS) Short-Term Postdoctoral Fellowship for Dr. Jason Sandahl. He will be collaborating with Professor Hiroshi Ueda at Hokkaido University in Japan. They will undertake a study on the functional labeling of olfactory receptor neurons. There are two main objectives of this research. The first is to further develop techniques that will visually label (and identify) stimulated olfactory neurons in salmon, including neurons of the olfactory epithelium (the nose), and the olfactory bulb and telencephalon (the forebrain). The second objective is to use these methods to identify the targets of three pesticides that are neurotoxic to the salmon olfactory system and are believed to affect specific regions of the olfactory pathway. The researchers will develop two labeling techniques. The first utilizes a compound called agmatine, which is a relatively small molecule that is able to permeate certain ion channels once they have been activated. This involves presenting the fish with an odorant (which activates the neurons) while simultaneously presenting the nose with agmatine. The second labeling technique was developed recently which utilizes a compound called FM1-43 in which the labeling is very rapid, and can be performed repeatedly in intact animals. They have chosen three model toxins to investigate, with each toxin believed to target a different region of the olfactory pathway. The toxins are Copper, Esfenvalerate and Chlorpyrifos. The project brings together the efforts of two laboratories that have complementary expertise and research capabilities. The research is expected to further knowledge in both fish biology and environmental toxicology. The anticipated outcomes of this collaborative research include the development of functional labeling techniques in the salmon olfactory system and identification of targets within the olfactory system of three common toxins. This project will offer a good opportunity to join efforts between the two countries. Through the exchange of ideas and technology, this project will broaden our base of basic knowledge and promote international understanding and cooperation. Results of the research will be disseminated at scientific meetings and in scientific journals.

0343958 Sandahl该奖项支持为期一年的日本科学促进协会（JSPS）短期博士后奖学金博士。 他将与日本北海道大学的上田教授合作。 他们将进行嗅觉受体神经元功能标记的研究。 本研究有两个主要目标。 首先是进一步开发技术，将视觉标记（和识别）刺激的嗅觉神经元在鲑鱼，包括神经元的嗅上皮（鼻子），嗅球和端脑（前脑）。 第二个目标是使用这些方法来确定三种农药的目标，这些农药对鲑鱼嗅觉系统具有神经毒性，并被认为会影响嗅觉通路的特定区域。 研究人员将开发两种标记技术。 第一种方法使用一种叫做胍丁胺的化合物，胍丁胺是一种相对较小的分子，一旦被激活，它就能够渗透某些离子通道。 这包括向鱼提供气味剂（激活神经元），同时向鼻子提供胍丁胺。 第二种标记技术是最近开发的，它利用了一种称为FM 1 -43的化合物，其中标记非常快速，并且可以在完整的动物中重复进行。 他们选择了三种模型毒素进行研究，每种毒素都被认为针对嗅觉通路的不同区域。 有毒物质是铜、氰戊菊酯和毒死蜱。 该项目汇集了两个具有互补专业知识和研究能力的实验室的努力。 这项研究有望进一步加深鱼类生物学和环境毒理学方面的知识。 这项合作研究的预期成果包括开发鲑鱼嗅觉系统中的功能标记技术，并确定三种常见毒素的嗅觉系统中的目标。 该项目将为两国共同努力提供一个很好的机会。 通过交流思想和技术，该项目将扩大我们的基础知识，促进国际理解与合作。 研究结果将在科学会议和科学期刊上传播。