Konfokales Mikroskop mit spektraler Detektion für Langzeitaufnahmen am lebenden Objekt

建立在扫描显微镜上的体内成像在过去几年中一直是我们研究的一个据点。除了我们自己的研究进展的需要外，我们所另外两个实验室的附加项目清单说明了对这种显微镜的迫切需要。在lm Neuenheimer费尔德”的校园里，大多数不同的共焦显微镜都是根据用户的要求量身定制的。所有这些共聚焦显微镜都在密集使用，没有免费的能力，让我们的研究继续下去。由于突变体在先进的实验胚胎学中的使用，我们需要许多和长（24- 72小时）的成像会话。这可以部分地通过多视图选项来克服，该选项允许部分地并行化分析，因此非常具有成本效益。为了可视化和跟踪视网膜细胞之间接触的形成，我们需要穿透>200 µm进入眼睛和视网膜组织，覆盖>15 µm的z体积，同时高速成像以充分跟踪神经元生长的动力学。

In vivo imaging building on scanning microscopy has been a stronghold of our research in the past years. Besides the requirement for the progress of our own research, the list of attached projects from two more laboratories in our institute states the immanent need for this microscope. Most of the different confocal microscopes on the campus lm Neuenheimer Feld” have been tailored to the requirements of the users. All these confocal microscopes are in intensive use and do not have free capacities to allow a continuation of our research. Due to the use of mutants in advanced experimental embryology we require many and long (24-72hrs) imaging sessions. This can in part be overcome by the multiview option that allows in part to parellelize the analysis and is thus very cost effective. To visualize and follow the formation of contacts between cells in the retina we need to penetrate >200 µm into the eye and retinal tissue, cover >15 µm of z-volume, while imaging at a high speed to adequately follow dynamics of neuronal outgrowths.