Konfokales Mikroskop mit spektraler Detektion für Langzeitaufnahmen am lebenden Objekt

在过去的几年里，基于扫描显微镜的体内成像一直是我们研究的一个据点。除了我们自己的研究进度的要求外，我们研究所另外两个实验室所附的项目清单也说明了对这台显微镜的内在需求。“纽恩海默菲尔德”校园里的大多数不同的共聚焦显微镜都是根据用户的要求量身定制的。所有这些共聚焦显微镜都在密集使用，没有自由的能力，以允许我们的研究继续。由于在高级实验胚胎学中使用突变体，我们需要许多长时间（24-72小时）的成像会议。这可以通过多视图选项部分地克服，多视图选项允许部分地并行分析，因此非常具有成本效益。为了可视化和跟踪视网膜中细胞之间接触的形成，我们需要穿透>200µm进入眼睛和视网膜组织，覆盖>15µm的z-体积，同时高速成像以充分跟踪神经元生长的动态。

In vivo imaging building on scanning microscopy has been a stronghold of our research in the past years. Besides the requirement for the progress of our own research, the list of attached projects from two more laboratories in our institute states the immanent need for this microscope. Most of the different confocal microscopes on the campus lm Neuenheimer Feld” have been tailored to the requirements of the users. All these confocal microscopes are in intensive use and do not have free capacities to allow a continuation of our research. Due to the use of mutants in advanced experimental embryology we require many and long (24-72hrs) imaging sessions. This can in part be overcome by the multiview option that allows in part to parellelize the analysis and is thus very cost effective. To visualize and follow the formation of contacts between cells in the retina we need to penetrate >200 µm into the eye and retinal tissue, cover >15 µm of z-volume, while imaging at a high speed to adequately follow dynamics of neuronal outgrowths.