RUI: Proteins as Guidance for Regioselective Modification of Steroids and Disaccharides

RUI：蛋白质作为类固醇和二糖区域选择性修饰的指导

• 批准号: 0404479
• 负责人: Sean Liu
• 金额: $ 4.88万
• 依托单位: Cal Poly Pomona Foundation, Inc.
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-28 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0404479&HistoricalAwards=false
• 关键词: RUI; Proteins; Guidance; Regioselective; Modification

The focus of this research is to develop a methodology for conferring regioselectivity in organic syntheses through specific binding of substrates to biological macromolecules. To demonstrate the feasibility of this approach, two projects with strong application potential will be undertaken. First, bovine serum albumin (BSA) will be used to guide the regioselective reduction of steroids. Second, a lectin (e.g., concanavalin A) will be used to guide the regioselective modification of disaccharides such as lactose and melibiose. These regioselectivities are based on the proteins' specific and unique binding properties. This new method may be useful in the development of new modified steroids and carbohydrates of commercial importance. Undergraduate students will be involved at all stages of this research in a team environment, from experimental design, synthesis, instrumental operation, to data collection and analysis. This not only trains them technical skills in biochemistry and organic chemistry, but also boosts their interest in pursuing research careers in these fields.

本研究的重点是开发一种通过底物与生物大分子的特异性结合赋予有机合成区域选择性的方法。为了证明这种方法的可行性，将开展两个具有强大应用潜力的项目。首先，牛血清白蛋白（BSA）将用于指导类固醇的区域选择性减少。其次，凝集素（例如伴刀豆球蛋白 A）将用于指导二糖（例如乳糖和蜜二糖）的区域选择性修饰。这些区域选择性基于蛋白质的特异性和独特的结合特性。这种新方法可能有助于开发具有商业重要性的新型修饰类固醇和碳水化合物。本科生将在团队环境中参与这项研究的所有阶段，从实验设计、合成、仪器操作到数据收集和分析。这不仅训练了他们在生物化学和有机化学方面的技术技能，而且增强了他们在这些领域从事研究事业的兴趣。