Developing a Combined Mass Spectrometry-Chemometrics Approach to Study Macromolecular Large Scale Dynamics and Association

开发质谱-化学计量相结合的方法来研究大分子大规模动力学和关联

• 批准号: 0406302
• 负责人: Igor Kaltashov
• 金额: $ 35.75万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0406302&HistoricalAwards=false
• 关键词: Developing; Combined; Mass; Spectrometry; Chemometrics

Professor Igor Kaltashov and Dr. Stephen Eyles of the University of Massachusetts Amherst are supported by the Analytical and Surface Chemistry Program to detect and quantify multiple protein conformations present in solution, and to study protein assembly. The idea is to utilize chemometric tools such as factor analysis to interpret protein ion charge state distributions observed in electrospray ionization (ESI) mass spectra. Two processes will be studied in detail: (i) the assembly of mammalian hemoglobin and(ii) the Tanford transition and dimerization of beta-lactoglobulin. If the techniques to be developed in this project are successful, they will allow more useful information on conformations to be obtained from experimental ESI data and may simplify the process of extracting information from the data.Electrospray Ionization mass spectrometry is a major method for analyzing proteins, and is highly used in proteomics. This project has high potential for making contributions in understanding the roles of protein dynamics in protein function. Students would be well-trained in mass spectrometry and its applications to biophysics of proteins.

马萨诸塞州阿默斯特大学的Igor Kaltashov教授和Stephen Eyles博士得到了分析和表面化学项目的支持，以检测和定量溶液中存在的多种蛋白质构象，并研究蛋白质组装。 我们的想法是利用化学计量学工具，如因子分析来解释蛋白质离子的电荷状态分布观察到的电喷雾电离（ESI）质谱。 将详细研究两个过程：（i）哺乳动物血红蛋白的组装和（ii）Tanford转变和β-乳球蛋白的二聚化。 如果本项目所开发的技术能够成功，将使我们能够从实验的ESI数据中获得更多有用的构象信息，并简化从数据中提取信息的过程。电喷雾质谱是分析蛋白质的主要方法，在蛋白质组学中应用广泛。 该项目在理解蛋白质动力学在蛋白质功能中的作用方面具有很高的潜力。 学生将在质谱及其在蛋白质生物物理学中的应用方面得到良好的培训。