BE/GEN-EN: Elucidating Mechanisms Responsible for the Persistence of Microorganisms in Drinking Water Distribution Systems via DNA Microarray Technology

0412618 Raskin尽管存在消毒剂残留物，但在饮用水分配系统中观察到微生物的生长和管道表面生物膜的形成。由于可能存在致病物种，这是一个公共卫生问题。本计画拟利用基因组DNA微阵列技术，探讨消毒残留物存在下微生物持续存在的机制。具体而言，这项工作将：（1）建立应用DNA微阵列技术研究环境微生物群落中特定微生物基因组响应的方法;（2）研究大肠杆菌和鸟分枝杆菌在纯培养和模拟分布系统的复杂群落中的全基因组转录谱;（3）分析这些表达谱以鉴定消毒剂暴露的响应特征模式，（4）描述选定的基因在饮用水分配系统中对消毒剂的抗性和生存方面的作用。这个项目的教育计划有很好的文件记录。每年将在该项目下培训三名研究生和两名本科生。 此外，还将为初中和高中教师举办讲习班。

0412618 RaskinGrowth of microorganisms and formation of biofilms on pipe surfaces is observed in drinking water distribution systems despite the presence of disinfectant residuals. Because of the possible presence of pathogenic species, this represents a public health concern. This project proposes to investigate the mechanism of microorganism persistence in the presence of disinfection residuals using genome-based DNA micro-arrays to examine gene expression patterns. Specifically, the work will: (1) Develop a method for the application of DNA micro-array technology for the study of specific microbial genome responses in environmental microbial communities, (2) Investigate whole genome transcription profiles of Escherichia coli and Mycobacterium avium in pure culture, and in the complex communities of a simulated distribution system, (3) Analyze these expression profiles to identify a pattern of responses characteristic of disinfectant exposure, (4) Characterize the role of selected genes in resistance to disinfectants and survival in drinking water distribution systems.The educational plan for this project is well documented. Three graduate students and two undergraduates per year will be trained under this project. In addition, workshops for middle and high school teachers will be developed.