BE/GENEN: Global Transcriptional Responses in Nitrogen Cycling and Nutrient Removal Processes and Development of Supplemental Instructional Workshops

0412711 ARP本提案的重点是用于废水处理的微生物系统中的一个重要问题。被选中进行研究的模式生物是唯一一种具有测序基因组的氨氧化细菌。将对浮游和生物膜培养物的转录反应进行评估。这种微生物的报告菌株也将被产生，可能用于污水处理厂，作为潜在有害条件的生物传感器。这项拟议的研究将使用硝化细菌N.uropaea作为环境压力对基因表达影响的模型。这将通过完整的基因组序列和随后全基因组微阵列的出现而实现。目标是确定在废水系统中作为压力标志的基因。如果成功，这种方法可以应用于其他环境系统。教育计划制定得很好，包括研究生的跨学科培训和高中生的参与。将为高中和社区大学教师开发和提供讲习班。

0412711 ArpThis proposal is focused on an important problem in microbial systems used for wastewater treatment. The model organism selected for study is the only ammonia-oxidizing bacterium with a sequenced genome. Transcriptional responses would be evaluated for both planktonic and biofilm cultures. Reporter strains of this organism would also be generated for potential use in wastewater treatment plants as biosensors of potentially deleterious conditions. The proposed research would use the nitrifying bacterium N. europaea as a model for the effects of environmental stress on gene expression. This would be enabled by the complete genome sequence and the subsequent availability of whole genome microarrays. The goal is to identify genes that would serve as markers of stress in wastewater systems. If successful, this approach could be applied to other environmental systems.The educational plan is well developed, including interdisciplinary training of graduate students and involving high school students. Workshops are to be developed and offered to high school and community college teachers.