BE/GENEN: Global Transcriptional Responses in Nitrogen Cycling and Nutrient Removal Processes and Development of Supplemental Instructional Workshops

[0412711 . arp]本提案关注的是用于废水处理的微生物系统中的一个重要问题。选择的模式生物是唯一基因组测序的氨氧化细菌。将对浮游生物和生物膜培养的转录反应进行评估。这种生物的报告菌株也将被产生，作为潜在有害条件的生物传感器，在废水处理厂中有潜在的用途。该研究将利用硝化细菌N. europaea作为环境胁迫对基因表达影响的模型。这将通过完整的基因组序列和随后可用的全基因组微阵列实现。目标是识别出可以作为废水系统压力标记的基因。如果成功，这种方法可以应用于其他环境系统。教育计划很完善，包括研究生的跨学科培训和高中生的参与。将为高中和社区大学教师开设讲习班。

0412711 ArpThis proposal is focused on an important problem in microbial systems used for wastewater treatment. The model organism selected for study is the only ammonia-oxidizing bacterium with a sequenced genome. Transcriptional responses would be evaluated for both planktonic and biofilm cultures. Reporter strains of this organism would also be generated for potential use in wastewater treatment plants as biosensors of potentially deleterious conditions. The proposed research would use the nitrifying bacterium N. europaea as a model for the effects of environmental stress on gene expression. This would be enabled by the complete genome sequence and the subsequent availability of whole genome microarrays. The goal is to identify genes that would serve as markers of stress in wastewater systems. If successful, this approach could be applied to other environmental systems.The educational plan is well developed, including interdisciplinary training of graduate students and involving high school students. Workshops are to be developed and offered to high school and community college teachers.