Evolution of Function of Histone-like HU Proteins
组蛋白样 HU 蛋白的功能进化
基本信息
- 批准号:0414875
- 负责人:
- 金额:$ 39.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-08-15 至 2008-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The bacterial histone-like protein HU has been primarily characterized in Escherichia coli. In addition to its role in genomic DNA compaction, E. coli HU has also been shown to participate in processes such as DNA repair and recombination. However, the range of DNA-binding properties ascribed to HU orthologs in vitro suggest that they serve distinct roles in vivo. A comparative approach to identifying molecular determinants of substrate specificity is implemented. The hypothesis is that the two kinks imposed by HU proteins are 'hinges' that must be secured, and that preference for distorted DNA occurs only if stabilizing contacts are insufficient to secure the hinges in perfect duplex DNA. Site-directed mutagenesis and electrophoretic techniques will be used to measure binding affinity, substrate specificity and site size. Proteins will include HU from Bacillus subtilis and from the hyperthermophile Thermotoga maritima. The hypothesis will also be considered that wrapping of DNA about the surface of HU proteins requires disruption of surface salt bridges. This hypothesis will be evaluated experimentally using the HU homolog TF1, a sequence-specific homolog encoded by a Bacillus bacteriophage, and HU from T. maritima by systematically modifying charged surface residues and measuring binding constants and their salt-dependence. This program focuses on integrating research and teaching by offering the opportunity for both graduate and undergraduate students to acquire research experience and proficiency in techniques associated with analysis of protein-nucleic acid interactions. Graduate students will be integral to developing these research projects, and minority undergraduate students will continue to contribute to related projects.
细菌组蛋白样蛋白HU已主要在大肠杆菌中表征。除了在基因组DNA压缩中的作用外,E.大肠杆菌HU也被证明参与DNA修复和重组等过程。然而,归因于HU直系同源物在体外的DNA结合特性的范围表明它们在体内发挥不同的作用。一个比较的方法来确定底物特异性的分子决定因素。该假说认为,HU蛋白所施加的两个扭结是必须固定的“铰链”,并且只有在稳定接触不足以固定完美双链体DNA中的铰链时,才会发生对扭曲DNA的偏好。定点诱变和电泳技术将用于测量结合亲和力、底物特异性和位点大小。蛋白质将包括来自枯草芽孢杆菌和来自超嗜热海栖热袍菌的HU。该假设也将被认为是DNA围绕HU蛋白表面的包裹需要破坏表面盐桥。这一假设将使用HU同源物TF 1(一种由芽孢杆菌噬菌体编码的序列特异性同源物)和来自T. maritima通过系统地修改带电的表面残基和测量结合常数及其盐依赖性。该计划的重点是整合研究和教学,为研究生和本科生提供机会,以获得研究经验和熟练掌握与蛋白质-核酸相互作用分析相关的技术。研究生将是开发这些研究项目不可或缺的,少数民族本科生将继续为相关项目做出贡献。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Anne Grove其他文献
Activity of the mammalian DNA transposon piggyBat from Myotis lucifugus is restricted by its own transposon ends
蝙蝠(Myotis lucifugus)哺乳动物 DNA 转座子 piggyBat 的活性受到其自身转座子末端的限制
- DOI:
10.1038/s41467-024-55784-9 - 发表时间:
2025-01-07 - 期刊:
- 影响因子:15.700
- 作者:
Alison B. Hickman;Laurie Lannes;Christopher M. Furman;Christina Hong;Lidiya Franklin;Rodolfo Ghirlando;Arpita Ghosh;Wentian Luo;Parthena Konstantinidou;Hernán A. Lorenzi;Anne Grove;Astrid D. Haase;Matthew H. Wilson;Fred Dyda - 通讯作者:
Fred Dyda
Control of DNA end resection by yeast Hmo1p affects efficiency of DNA end-joining
- DOI:
10.1016/j.dnarep.2017.03.002 - 发表时间:
2017-05-01 - 期刊:
- 影响因子:
- 作者:
Arvind Panday;LiJuan Xiao;Ashish Gupta;Anne Grove - 通讯作者:
Anne Grove
Anne Grove的其他文献
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{{ truncateString('Anne Grove', 18)}}的其他基金
Purine catabolites as inducers of bacterial gene expression
嘌呤分解代谢物作为细菌基因表达的诱导剂
- 批准号:
2153410 - 财政年份:2022
- 资助金额:
$ 39.29万 - 项目类别:
Standard Grant
Purine catabolites as inducers of bacterial gene expression
嘌呤分解代谢物作为细菌基因表达的诱导剂
- 批准号:
1714219 - 财政年份:2017
- 资助金额:
$ 39.29万 - 项目类别:
Standard Grant
Evolution of Function of Bacterial PecS Family Transcription Factors
细菌PecS家族转录因子功能的进化
- 批准号:
1515349 - 财政年份:2015
- 资助金额:
$ 39.29万 - 项目类别:
Standard Grant
Evolution of Function of Bacterial Nucleoid-Associated Proteins
细菌核相关蛋白的功能进化
- 批准号:
1051610 - 财政年份:2011
- 资助金额:
$ 39.29万 - 项目类别:
Continuing Grant
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