Functional Genomics of Early Events in Ectomycorrhizal Symbiosis: The Fungal Perspective"

外生菌根共生早期事件的功能基因组学：真菌视角”

• 批准号: 0421326
• 负责人: Gopi Podila
• 金额: --
• 依托单位: University of Alabama in Huntsville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0421326&HistoricalAwards=false
• 关键词: Functional; Genomics; Early; Events; Ectomycorrhizal

Functional Genomics of Early Events in Ectomycorrhizal Symbiosis: A fungal PerspectiveEctomycorrhizal fungi colonize roots and establish beneficial relationships with a wide variety of tree species. These fungi extend the absorptive capabilities of the root system, improve the host plant's abilities to tolerate biological and environmental stresses and support plant health under conditions of nutritional deficiency. The dynamic signaling and cross-talk events and genes involved during the pre-infection stage, before the physical contact is established between the fungus and the plant host, determine the direction and ultimate success of the formation of ectomycorrhizal association. The PI's lab has identified and well characterized an interaction-specific and signaling related LbRAS gene and also identified expressed sequence tags (ESTs) coding for GTPases and receptor kinases from the ectomycorrhizal fungus Laccaria bicolor. The PI will use these genes and yeast two-hybrid interactions to characterize the genes and begin to unravel signaling pathways involved in the establishment and maintenance of ectomycorrhiza by L. bicolor. This project also involves development of new tools for the functional genomic studies of ectomycorrhizal symbiosis using Agrobacterium-mediated gene transfer into L.bicolor. This work provides educational and research-training opportunities for both undergraduate and postdoctoral students to integrate various functional genomic approaches and develop tools to study a very important yet understudied beneficial interaction.

外生菌根共生早期事件的功能基因组学：真菌的观点外生菌根真菌定殖于根部，并与多种树种建立有益的关系。这些真菌扩展了根系的吸收能力，提高了宿主植物耐受生物和环境胁迫的能力，并在营养缺乏的条件下支持植物健康。在真菌与植物宿主之间建立物理接触之前，在感染前阶段所涉及的动态信号和串扰事件和基因决定了外生菌根联合体形成的方向和最终成功。PI的实验室已经鉴定并充分表征了相互作用特异性和信号相关的LbRAS基因，并且还鉴定了编码来自外生菌根真菌双色蜡蘑（Laccaria bicolor）的GTP酶和受体激酶的表达序列标签（EST）。 PI将使用这些基因和酵母双杂交相互作用来表征基因，并开始解开参与L.双色的 该项目还涉及开发新的工具，利用农杆菌介导的基因转移到L.双色外生菌根共生的功能基因组研究。这项工作为本科生和博士后学生提供了教育和研究培训机会，以整合各种功能基因组方法并开发工具来研究非常重要但尚未充分研究的有益相互作用。