MRI: Acquisition of a Proteome Analyzer

MRI：购买蛋白质组分析仪

• 批准号: 0421537
• 负责人: Jamil Momand
• 金额: $ 34.73万
• 依托单位: California State L A University Auxiliary Services Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0421537&HistoricalAwards=false
• 关键词: MRI; Acquisition; Proteome; Analyzer

A grant has been awarded to California State University-Los Angeles (Cal State L.A.) under the direction of Dr. Jamil Momand for partial support of a Proteome Analyzer. In recent years it has been established that each human can express 25,000 different proteins from the genome. Using this knowledge, the scientific community has been able to increase its understanding of how the timing and expression levels of these genes affect the behavior of cells yet the exact timing of the expression of genes during development and the response of the genes to environmental toxins is not completely known. To understand these secrets one needs to analyze the proteome-the complete set of proteins expressed in an organism. Isolated proteins will be fragmented and the masses of the fragments will be measured. The precise measurement of the fragment masses is, in fact, a fingerprint of the protein. Public databases accessible through the internet will be used identify the protein based on the fingerprint. The goal of the project is to increase the understanding of how protein expression and modification is altered by environmental pollutants, drugs, natural development, diseases and genetic mutations. The Proteome Analyzer is a powerful tool that can identify each protein and measure subtle changes in each protein's behavior. The Proteome Analyzer will be used by seven investigators performing research with undergraduates and Masters Degree students at Cal State LA. For example, Dr. Momand's laboratory will identify proteins that increase expression in response to high levels of hydrogen peroxide. Dr. Vellanoweth will identify plant transcription factors that bind to DNA elements and identify proteins associated with the bolting process in flower development. Dr. LaPolt's laboratory will examine differences in patterns of protein expression in the pituitary glands of young cyclic versus middle-aged acyclic persistent-estrous female rats. Dr. Krug's laboratory will compare the proteins expressed during development in the marine mollusc Alderia modesta. The Proteome Analyzer will constitute part of a new laboratory course that will train undergraduates in the use of three pieces of equipment. In this course students will learn how to use the Proteome Analyzer, a new 600 MHz NMR, and a new Electron Spin Resonance machine.Cal State LA is a Minority-Serving Institution that has made a major impact on the educational and research experiences of minorities. Cal State LA graduates a very large number of students with Hispanic ancestry in the US. It has an excellent reputation in providing bridges to top-rated Ph.D. programs for its undergrad and graduate students. The acquisition of the Proteome Analyzer will give its motivated faculty a research tool to enable them to perform cutting-edge research. Because the Proteome Analyzer will be incorporated into the curriculum of a new laboratory course, it will provide valuable training to all Cal State LA students pursuing molecular life science degrees.

在 Jamil Momand 博士的指导下，加州州立大学洛杉矶分校 (Cal State L.A.) 获得了一笔赠款，用于蛋白质组分析仪的部分支持。近年来，已经确定每个人可以表达基因组中的 25,000 种不同的蛋白质。 利用这些知识，科学界已经能够加深对这些基因的时间和表达水平如何影响细胞行为的理解，但发育过程中基因表达的确切时间以及基因对环境毒素的反应尚不完全清楚。 为了了解这些秘密，我们需要分析蛋白质组——有机体中表达的完整蛋白质组。 分离的蛋白质将被打碎，并测量碎片的质量。 事实上，片段质量的精确测量是蛋白质的指纹。 通过互联网访问的公共数据库将用于根据指纹识别蛋白质。 该项目的目标是加深对环境污染物、药物、自然发育、疾病和基因突变如何改变蛋白质表达和修饰的了解。 蛋白质组分析仪是一个强大的工具，可以识别每种蛋白质并测量每种蛋白质行为的细微变化。 蛋白质组分析仪将由七名研究人员使用，与加州州立大学洛杉矶分校的本科生和硕士学位生进行研究。 例如，莫曼德博士的实验室将鉴定出因高水平过氧化氢而增加表达的蛋白质。 Vellanoweth 博士将鉴定与 DNA 元件结合的植物转录因子，并鉴定与花朵发育中抽苔过程相关的蛋白质。 拉波尔特博士的实验室将检查年轻周期性发情雌性大鼠与中年非周期性持续发情雌性大鼠垂体中蛋白质表达模式的差异。 克鲁格博士的实验室将比较海洋软体动物 Alderiamodeta 发育过程中表达的蛋白质。 蛋白质组分析仪将构成新实验室课程的一部分，该课程将培训本科生如何使用三台设备。 在本课程中，学生将学习如何使用蛋白质组分析仪、新型 600 MHz NMR 和新型电子自旋共振机。加州州立大学洛杉矶分校是一所为少数族裔服务的机构，对少数族裔的教育和研究经历产生了重大影响。 加州州立大学洛杉矶分校在美国培养了大量具有西班牙裔血统的学生。 它在为顶级博士提供桥梁方面享有盛誉。为其本科生和研究生提供课程。 收购蛋白质组分析仪将为积极进取的教师提供一个研究工具，使他们能够进行前沿研究。 由于蛋白质组分析仪将被纳入新实验室课程的课程中，因此它将为所有攻读分子生命科学学位的加州州立大学洛杉矶分校学生提供有价值的培训。