The role of HCN channels in the septo-hippocampal circuit (B05)

内侧隔在海马体产生θ波节律中起着至关重要的作用，对学习和记忆至关重要。在这个建议中，我们将讨论内隔膜的HCN通道对这种节律是否重要。我们将特异性敲除小鼠中间隔神经元不同亚群（全局、gaba能和谷氨酸能）中的HCN2。我们将研究HCN2的缺失如何改变急性脑切片（目的1）和完整的中隔-海马准备（目的2）中中隔神经元的放电模式。在目标#3中，我们将使用麻醉小鼠的深度记录、脑电图遥测和行为实验来表征HCN2的体内功能。

The medial septum has an essential role for theta rhythm generation in the hippocampus and is critical for learning and memory. In this proposal we will address the question whether HCN channels in the medial septum are important for this rhythm. We will specifically knock out HCN2 in distinct subsets of medial septal neurons (global, GABAergic and glutamatergic) in the mouse. We will investigate how loss of HCN2 alters firing pattern in medial septal neurons in acute brain slices (aim #1) and in intact septo-hippocampal preparation (aim #2). In aim #3 we will characterize in vivo function of HCN2 using depth recording in anesthesized mice, EEG telemetry and behavioral experiments.