CAREER: Diversity Directed Fluorescence Sensor Development

职业：多样性定向荧光传感器开发

• 批准号: 0449139
• 负责人: Young-Tae Chang
• 金额: $ 50.55万
• 依托单位: New York University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0449139&HistoricalAwards=false
• 关键词: CAREER; Diversity; Directed; Fluorescence; Sensor

Professor Young-Tae Chang of New York University is supported by the Analytical and Surface Chemistry CAREER Program to create combinatorial libraries of indicators for fluorescent staining of organelles and macro-molecular assemblies. The PI will synthesize a library of new fluorescent styryl dyes that will be investigated as fluorescent probes for biological localization in cell organelles, selective response to biological macromolecules, and for the development of an artificial "tongue" approach for low response small molecule analytes. The PI has already prepared a library of styryl dyes and developed a mathematical model for color prediction. This work will expand this library by increasing the diversity of structural elements around the styryl moiety. He will do this through the same solution phase chemistry already utilized, as well as develop new solid-state phase chemistry. The key idea behind this research work is the so-called Diversity Directed Sensor approach, which holds that the various structural elements located around the fluorescent styryl dye moiety will vary the selectivity of noncovalent interactions between the dyes and cell organelles or biological macromolecules. The resulting recognition elements will have applications in various sensing and chemical imaging applications.The goal to chemically image a living cell is a grand challenge. Production of small molecules that cause varying parts of the cell to "light up" under a microscope hold one key to revealing the chemical makeup of the cell. This research will expand our capacity to produce these dye molecules. One application is the detection of amyloids in neural tissue, which are thought to cause diseases such as Alzheimer's. The PI engages high school, college and graduate students in this interdisciplinary research, enabling them to publish in the scientific literature.

纽约大学的Young-Tae Chang教授得到了分析和表面化学CAREER计划的支持，以创建用于细胞器和大分子组装体荧光染色的指示剂组合库。PI将合成一个新的荧光苯乙烯基染料库，这些染料将作为荧光探针进行研究，用于细胞器中的生物定位，对生物大分子的选择性响应，以及开发用于低响应小分子分析物的人工“舌头”方法。PI已经准备了苯乙烯基染料库，并开发了颜色预测的数学模型。这项工作将通过增加苯乙烯基部分周围结构元件的多样性来扩展该文库。他将通过已经使用的相同溶液相化学方法来实现这一目标，并开发新的固态相化学方法。这项研究工作背后的关键思想是所谓的多样性定向传感器方法，该方法认为位于荧光苯乙烯基染料部分周围的各种结构元件将改变染料与细胞器或生物大分子之间的非共价相互作用的选择性。由此产生的识别元件将应用于各种传感和化学成像应用。对活细胞进行化学成像的目标是一个巨大的挑战。 在显微镜下，小分子的产生使细胞的不同部分“发光”，这是揭示细胞化学组成的关键。 这项研究将扩大我们生产这些染料分子的能力。 一个应用是检测神经组织中的淀粉样蛋白，这被认为是导致阿尔茨海默氏症等疾病的原因。 PI使高中，大学和研究生参与这项跨学科研究，使他们能够在科学文献中发表。