International Research Fellowship Program: Detection and Quantification of Trace Organic Compounds in Breath: Consideration of Sensor Array Technologies & Sampling Methodologies

国际研究奖学金计划：呼吸中痕量有机化合物的检测和定量：传感器阵列技术的考虑

• 批准号: 0502247
• 负责人: Mary-Grace Danao
• 金额: --
• 依托单位: Danao Mary-Grace C
• 依托单位国家: 美国
• 项目类别: Fellowship
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0502247&HistoricalAwards=false
• 关键词: International; Research; Fellowship; Program; Detection

0502247DanaoThe International Research Fellowship Program enables U.S. scientists and engineers to conduct three to twenty-four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad.This award will support a twenty-one-month research fellowship by Dr. Mary-Grace C. Danao to work with Dr. Todd Mottram at Silsoe Research Institute and David Cullen at Cranfield University in Bedfordshire, United Kingdom.Breath-monitoring is a non-invasive, safe, and easy approach to determining the general health status of humans and mammals. Current analytical methods used for breath analysis include gas chromatography, mass spectrometry, infrared analysis, and electronic noses - which have varying specificity to detect compounds with varying sensitivity in the parts per million or parts per billion levels. However, the size, cost, and complexity of these instruments make them unsuitable for routine in situ monitoring. The Pis will investigate the development of a sampling system and specific biosensor array to detect and analyse key analytes (biomarkers) in mammalian breath and breath condensate samples. The research aims to provide generic techniques for breath sampling and analysis and it will be implemented by an exemplar. A prime exemplar is identifying key breath markers of respiratory stress in horses. Specific aims of the research program include (1) identifying and quantifying volatile and non-volatile organic compounds in breath and breath condensate samples; (2) identifying biosensor technologies appropriate to the identified compounds, their dynamics, and cross-sensitivities of the sensors; (3) evaluating different extraction and pre-concentration techniques; (4) designing, building, and testing a bench-scale sensor array; (5) designing, building, and testing a laboratory breath output simulator to aid the development of a sensor array; and (6) validating the sensor array system using model and specific breath samples. This research program leads to a general understanding of sampling and sensing of trace organic compounds in breath and future development of non-invasive systems for breath-based analysis of biomarkers of disease, health, and biosecurity.

国际研究奖学金计划使美国科学家和工程师能够在国外进行三到二十四个月的研究。该计划的奖项提供了联合研究的机会，以及使用独特或互补的设施，专业知识和国外的实验条件。Danao与Silsoe研究所的托德莫特拉姆博士和英国贝德福德郡克兰菲尔德大学的大卫卡伦博士合作。呼吸监测是一种非侵入性、安全和简单的方法，可以确定人类和哺乳动物的一般健康状况。目前用于呼吸分析的分析方法包括气相色谱法、质谱法、红外分析法和电子鼻-它们具有不同的特异性，以检测具有不同灵敏度的化合物，灵敏度在百万分之一或十亿分之一水平。然而，这些仪器的尺寸、成本和复杂性使其不适合常规原位监测。Pis将研究开发一种采样系统和特定的生物传感器阵列，以检测和分析哺乳动物呼吸和呼吸冷凝物样本中的关键分析物（生物标志物）。该研究旨在提供呼吸采样和分析的通用技术，并将通过一个范例来实现。一个主要的例子是确定马呼吸应激的关键呼吸标志物。该研究计划的具体目标包括：（1）识别和量化呼吸和呼吸冷凝物样本中的挥发性和非挥发性有机化合物;（2）识别适合于所识别化合物的生物传感器技术，它们的动力学和传感器的交叉灵敏度;（3）评估不同的提取和预浓缩技术;（4）设计，构建和测试实验室规模的传感器阵列;（5）设计，构建和测试传感器阵列。（5）设计、构建和测试实验室呼吸输出模拟器以帮助传感器阵列的开发;以及（6）使用模型和特定呼吸样本来验证传感器阵列系统。该研究计划导致对呼吸中痕量有机化合物的采样和传感的一般理解，以及对疾病，健康和生物安全生物标志物的呼吸分析的非侵入性系统的未来发展。