RNA-protein-protein interactions of plant RNA editing factors

植物线粒体和质体中的RNA编辑过程需要几种蛋白质组分来有效地将靶RNA中的特定C核苷酸改变为U。PPR蛋白是一个独特的RNA序列基序附近的单个或几个编辑位点的识别因子。每个MORF蛋白影响许多位点，但它们的确切功能仍然是个谜。该项目旨在分析靶RNA、PPR和MORF蛋白之间的RNA-蛋白质和蛋白质-蛋白质相互作用，以更好地确定它们各自的功能及其在编辑体中的合作。此外，蛋白质-蛋白质交互式搜索将被用来搜索拟南芥中编辑体的其他组分，包括仍然未知的酶活性。

The RNA editing process in mitochondria and plastids of plants requires several protein components for efficient alteration of specific C nucleotides to U in target RNAs. PPR proteins are identifying factors for a unique RNA sequence motif near single or few editing sites. Each of the MORF proteins affect numerous sites, but their precise function is still enigmatic. This project aims to analyze RNA-protein and protein-protein interactions between the target RNAs, the PPR and the MORF proteins to better define their respective function and their cooperation in the editosome. Furthermore, protein-protein interactive searches will be employed to search for additional components of the editosome in Arabidopsis thaliana including the still unknown enzymatic activity.