Building Concepts for Succession: Indentifying Mechanisms for Ectomycorrhizal Fungus Succession and Developing Undergraduate Education/Research Opportunities

建立继承概念：确定外生菌根真菌的继承机制和开发本科教育/研究机会

• 批准号: 0516456
• 负责人: Ari Jumpponen
• 金额: $ 8.92万
• 依托单位: Kansas State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0516456&HistoricalAwards=false
• 关键词: Building; Concepts; Succession; Indentifying; Mechanisms

Communities of plants and fungi establish in severely disturbed sites (e.g. glacier forelands, volcanic deposit areas) in a fairly well described order of succession. Succession and its mechanisms are focal areas in ecology and a number of ecological theories explain the succession-related community change of plants and fungi. This proposal rests on a succession model for fungi that adopts from community assembly rules, which can be described as a set of admission rules that allow arrival and persistence of organisms in any ecosystem. The main goal of this program is to identify and assess the sources of organisms in a glacier forefront ecosystem and to identify the organisms that comprise the living (metabolically active fungal communities). To study this idea, we will employ novel molecular tools that focus on the protein-synthesizing ribosomes and the DNA that encodes them. Use of direct, simultaneous analysis of ribosomal RNA (rRNA; the building block of ribosomes) and the genes (rDNA) that encode rRNA will allow determining which organisms are present in early and late successional environments (present as rDNA) and which of them are active (present as both rRNA and rDNA). This proposal will experimentally assess sources of fungi in an assembly model for fungal succession. The proposed research will improve our understanding of the mechanisms behind the community change of fungi. The results will likely to be relevant as a background for planning restoration and conservation programs.

植物和真菌群落在受到严重干扰的地点（如冰川前陆、火山沉积区）以一个相当好的描述顺序建立。演替及其机制是生态学研究的热点，许多生态学理论解释了植物和真菌演替相关的群落变化。这一建议基于真菌的演替模型，该模型采用了群落组装规则，这可以被描述为一套允许生物在任何生态系统中到达和持续存在的准入规则。该计划的主要目标是识别和评估冰川前沿生态系统中生物的来源，并识别构成生物（代谢活跃的真菌群落）的生物。为了研究这个想法，我们将采用新颖的分子工具，专注于蛋白质合成核糖体和编码它们的DNA。使用直接的，同时分析核糖体RNA （rRNA；核糖体的组成部分）和编码rRNA的基因（rDNA）将允许确定哪些生物体存在于早期和晚期演替环境中（以rDNA的形式存在），哪些生物体是活跃的（以rRNA和rDNA的形式存在）。这一建议将在真菌演替的组装模型中实验评估真菌的来源。本研究将提高我们对真菌群落变化机制的认识。研究结果可能会成为规划修复和保护项目的相关背景。