Functions and Interactions of Essential PcsB in Pneumococcus Cell Wall Biosynthesis

肺炎球菌细胞壁生物合成中必需 PcsB 的功能和相互作用

• 批准号: 0516716
• 负责人: Malcolm Winkler
• 金额: $ 8.76万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0516716&HistoricalAwards=false
• 关键词: Functions; Interactions; Essential; PcsB; Pneumococcus

The PcsB protein is a conserved, critical and essential protein with murein hydrolyase like features that is present in the gram positive bacterium Streptococcus pneumoniae (pneumococcus). The long term goal of this project is to determine the functions and interactions of the PcsB protein in murein biosynthesis and cell shape determination. The following two aims are being addressed. First, the localization of the PcsB protein is being determined in wild-type and mutant bacteria by immunofluorescent approaches and phenotypes of pcsB mutants that may give insights into PcsB function(s) are being characterized. Second, the biochemical function(s) of PcsB and its interactions with other proteins, some of which may regulate its activity and binding to the cell surface, are being determined. This project will apply and refine genetic methods for use in S. pneumoniae, and provide new information and insights into the fundamental processes of peptidoglycan biosynthesis and architecture in gram positive bacteria. pneumococcus and other species of streptococcus. In addition, it will provide important new information about the links between murein biosynthesis and cell shape determination in spherically shaped bacteria. The project will contribute to the training of undergraduate and graduate students.

PcsB 蛋白是革兰氏阳性细菌肺炎链球菌（肺炎球菌）中存在的一种保守、关键和必需的蛋白，具有类似壁质水解酶的特征。该项目的长期目标是确定 PcsB 蛋白在胞壁质生物合成和细胞形状确定中的功能和相互作用。正在解决以下两个目标。首先，通过免疫荧光方法确定了野生型和突变细菌中 PcsB 蛋白的定位，并且正在表征可深入了解 PcsB 功能的 pcsB 突变体表型。其次，正在确定 PcsB 的生化功能及其与其他蛋白质的相互作用，其中一些蛋白质可能调节其活性和与细胞表面的结合。该项目将应用和完善用于肺炎链球菌的遗传方法，并为革兰氏阳性菌中肽聚糖生物合成和结构的基本过程提供新的信息和见解。肺炎球菌和其他种类的链球菌。此外，它将提供有关球形细菌中胞壁蛋白生物合成和细胞形状决定之间联系的重要新信息。该项目将有助于本科生和研究生的培养。