SBIR Phase I: A Lateral Field Excited Immunosensor for Pathogenic Bacteria in Drinking Water

这项小型企业创新研究第一阶段项目将展示横向场激发（LFE）声波免疫传感器检测饮用水中致病菌大肠杆菌的可行性。目前检测饮用水中大肠杆菌的方法有几个主要的缺点，包括时间和劳动力密集的样本采集和冗长的实验室分析，可能需要至少24小时。研究目标将是优化LFE电极的几何形状，从而在at切割的石英中获得最大的传感面积和有效的厚度剪切模式激发，并确定LFE免疫传感器检测非肠致病性大肠杆菌的灵敏度、选择性、响应时间和再现性，并将其与使用标准石英晶体微天平（QCM）获得的类似结果进行比较。第一阶段的工作将产生一种免疫传感器，它不仅直接适用于饮用水质量的原位测定，而且可能适用于环境安全、国土安全、农业和医学等领域的广泛应用。

This Small Business Innovation Research Phase I project will demonstrate the feasibility of a lateral field excited (LFE) acoustic wave immunosensor for the detection of the pathogenic bacteria, E. coli, in drinking water. Current methods of testing drinking water for E. coli have several major drawbacks, which include time- and labor-intensive sample collection and lengthy laboratory analyses that can take at least 24 hours. The research objectives will be to optimize the LFE electrode geometry resulting in a maximum sensing area and efficient excitation of the thickness shear mode in AT-cut quartz and to determine the LFE immunosensor sensitivity, selectivity, response time, and reproducibility for the detection of non-enteropathogenic E. coli and compare them to similar results obtained using the standard quartz crystal microbalance (QCM). The Phase I work will result in an immunosensor that is not only directly applicable for the in situ determination of drinking water quality but may also be applicable for a wide range of applications in areas such as environmental safety, homeland security, agriculture, and medicine.