NER: Protein chips of attoliter protein droplets on microelectrodes

这个项目将合成不同长度和不同组分比例的定制嵌段共聚物，测试几种感兴趣蛋白质的液滴生成。这种聚合物产生浓缩的蛋白质相，然后被交联并定位在电极上。溶剂可以被除去，因此可以进行预浓缩。作者在电极上显示了精确的定位，相当于信号放大。这些成分加在一起，将形成新型的2D蛋白质微芯片。计划中的工作需要合成一种聚合物，无论溶液中存在什么蛋白质，这种聚合物都会相分离，并在形成的液滴中捕获足够数量的每种蛋白质。第二个具体目标是开发在微电极上局部产生和沉积液滴的程序。

0609387VekilovThis project will synthesize tailored block copolymers with varying length and ratios between components, test droplet generation with several proteins of interest. The polymer creates concentrated protein phases and then is cross-linked and localized onto electrodes. The solvent can be removed and hence a pre-concentration follows. The authors show precise localization on electrodes, amounting to signal amplification. Together, the components will form novel 2D protein microchips. The planned work requires the synthesis of a polymer that will phase separate regardless of the proteins present in the solution and entrap sufficient quantities of each protein in the formed droplets. The second specific aim is to develope procedures for localized generation and deposition of the droplets over microelectrodes.