Towards a mechanistic framework of plant stem cell control (B01)

建立植物干细胞控制的机制框架（B01）

• 批准号: 178401084
• 金额: --
• 依托单位: Forschungsgruppe Stammzellbiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/178401084?language=en
• 关键词: Towards; mechanistic; framework; plant; stem

Project B01 will decode the mechanisms underlying the precise spatio-temporal control of gene ex-pression by the stem cell inducing WUSCHEL (WUS) transcription factor in the shoot apical meristem of the reference plant Arabidopsis thaliana. Based on our findings that stem cell function is dependent on WUS protein distribution, selective DNA binding, as well as differential response of individual cells to WUS, we will now elucidate the structural basis for the amazing ability of WUS to bind a diverse set of DNA motifs and to move from cell to cell. Complementing these mechanistic efforts, we will charac-terize the regulatory landscape encoded in trans by delineating the capacity of shoot meristem cells to respond to stem cell inductive WUS signals by single cell transcript profiling.

项目B 01将解码参考植物拟南芥茎尖分生组织中干细胞诱导WUSCHEL（WUS）转录因子精确时空控制基因表达的机制。基于我们的研究结果，即干细胞功能依赖于WUS蛋白分布，选择性DNA结合以及单个细胞对WUS的差异反应，我们现在将阐明WUS结合不同DNA基序并从细胞转移到细胞的惊人能力的结构基础。补充这些机制的努力，我们将character-pronounced的监管景观编码的反式描绘能力的茎分生组织细胞响应干细胞诱导WUS信号的单细胞转录谱。