MRI: Acquisition of a Beckman XL-I Analytical Ultracentrifuge

该奖项支持为一所大学购买一种用于蛋白质组表征的超离心机，该超离心机结合了扫描紫外线吸收和瑞利干涉光学系统，目前该大学没有分析性超离心机。这六名研究人员的研究项目涉及多种生物分子特征，如蛋白质-蛋白质复合物、蛋白质-脂质、蛋白质- dna /RNA和蛋白质寡聚物结构。分析性超离心将在不同的项目中使用，以解决有关上述相互作用的关键机制和管理问题。提出的研究的一个新颖方面是使用分析性超离心来检查氧化还原状态的变化如何影响蛋白质寡聚物行为、蛋白质- dna结合和蛋白质-脂质结合。除了推进个人研究项目外，超离心系统还将改善研究基础设施和学生的教育培训。超离心机将有利于生物化学实验室课程在本科和研究生水平，以及夏季本科生研究人员。

This award supports the acquisition of an ultracentrifuge for proteome characterization that combines scanning UV-absorption and Rayleigh interference optical systems, for a university that currently has no analytical ultracentrifuge. The six investigators have research programs involving a variety of biomolecular characterizations such as protein-protein complexes, protein-lipid, protein-DNA/RNA and protein oligomeric structures. Analytical ultracentrifugation will be used in the different projects to address key mechanistic and regulatory questions concerning the aforementioned interactions. One novel aspect of the proposed studies is the use of analytical ultracentrifugation to examine how changes in redox state influence protein oligomeric behavior, protein-DNA binding, and protein-lipid associations. In addition to advancing individual research projects, the ultracentrifuge system will improve the research infrastructure and educational training for students. The ultracentrifuge will benefit the biochemistry laboratory curriculum at both the undergraduate and graduate levels, as well as the summer undergraduate researchers.