MRI: Acquisition of a Beckman XL-I Analytical Ultracentrifuge

该奖项支持为一所目前没有分析超精密仪器的大学购买结合了扫描紫外吸收和瑞利干涉光学系统的蛋白质组表征超精密仪器。这六名研究人员的研究项目涉及各种生物分子表征，如蛋白质-蛋白质复合物、蛋白质-脂质、蛋白质-DNA/RNA和蛋白质寡聚体结构。在不同的项目中，将使用分析超浓缩技术来解决与上述相互作用有关的关键机制和监管问题。提出的研究的一个新的方面是使用分析超离心来研究氧化还原状态的变化如何影响蛋白质寡聚体的行为，蛋白质-DNA结合，和蛋白质-脂质协会。除了推进个人研究项目外，超级中心系统还将改善研究基础设施和学生的教育培训。该ultracenteligge将有利于生物化学实验室课程在本科和研究生水平，以及夏季本科研究人员。

This award supports the acquisition of an ultracentrifuge for proteome characterization that combines scanning UV-absorption and Rayleigh interference optical systems, for a university that currently has no analytical ultracentrifuge. The six investigators have research programs involving a variety of biomolecular characterizations such as protein-protein complexes, protein-lipid, protein-DNA/RNA and protein oligomeric structures. Analytical ultracentrifugation will be used in the different projects to address key mechanistic and regulatory questions concerning the aforementioned interactions. One novel aspect of the proposed studies is the use of analytical ultracentrifugation to examine how changes in redox state influence protein oligomeric behavior, protein-DNA binding, and protein-lipid associations. In addition to advancing individual research projects, the ultracentrifuge system will improve the research infrastructure and educational training for students. The ultracentrifuge will benefit the biochemistry laboratory curriculum at both the undergraduate and graduate levels, as well as the summer undergraduate researchers.