Molecular and functional analysis of trehalose metabolism during pea seed development

种子代谢必须与内源发育因子和外源环境因子协调。这被认为是由SnRK1、脱落酸和海藻糖（Tre）途径介导的糖信号传导完成的。tre3和tre6 - p是植物中次要的糖类，但它们是整合和协调代谢与发育的重要信号。在这个项目中，我们计划在豌豆种子发育过程中编码Tre代谢酶的基因的分子和功能特征。具体来说，我们希望研究种子中tre3代谢基因的组织和细胞特异性基因表达。基因表达的调控将在一组独特的转基因豌豆种子模型中进行监测，并确定其代谢状态的变化。这些基因的启动子活性调控将根据营养/激素处理进行分析。此外，我们希望通过寻找蛋白质相互作用伙伴来揭示II类trep合成酶的调控。最后，我们计划通过转基因抑制基因表达来分析豌豆种子发育过程中tre3代谢的功能。大肠杆菌trep合成酶和trep磷酸酶的异位表达将改变tre6 - p的水平。转基因种子将通过监测生长参数和种子成分进行表型分析。通过这个项目，我们期望对豌豆种子和作物种子的糖代谢和储存产物合成的调控有更深刻的认识。

Seed metabolism must be coordinated with endogenous developmental and exogenous environmental factors. This is thought to be performed by sugar signalling mediated by SnRK1, abscisic acid and the Trehalose (Tre) pathway. Tre and Tre-6-P are minor sugars in plants but represent important signals to integrate and coordinate metabolism with development. In this project, we are planning a molecular and functional characterisation of genes encoding Tre metabolising enzymes during pea seed development. In detail, we want to study tissue and cell-specific gene expression of Tre metabolism genes in seeds. Regulation of gene expression will be monitored in a set of unique transgenic pea seed models with defined changes of their metabolic state. Regulation of promoter activities from these genes will be analysed in response to nutrient/hormone treatments. In addition, we want to unravel the regulation of class II Tre-P synthases by searching for protein interacting partners. Finally, we are planning a functional analysis of Tre metabolism during pea seed development by transgenic inhibition of gene expression. Levels of Tre-6-P will be modified by ectopic expression of E. coli Tre-P synthase and Tre-P phosphatase. Transgenic seeds will be subjected to phenotypic analysis by monitoring growth parameters and seed composition. From this project, we expect a more profound insight into the regulation of sugar metabolism and storage product synthesis of pea seeds in particular and of crop seeds in general.