Molecular and functional analysis of trehalose metabolism during pea seed development

豌豆种子发育过程中海藻糖代谢的分子和功能分析

• 批准号: 178974956
• 负责人: Dr. Hans Weber
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/178974956?language=en
• 关键词: Molecular; functional; analysis; trehalose; metabolism

Seed metabolism must be coordinated with endogenous developmental and exogenous environmental factors. This is thought to be performed by sugar signalling mediated by SnRK1, abscisic acid and the Trehalose (Tre) pathway. Tre and Tre-6-P are minor sugars in plants but represent important signals to integrate and coordinate metabolism with development. In this project, we are planning a molecular and functional characterisation of genes encoding Tre metabolising enzymes during pea seed development. In detail, we want to study tissue and cell-specific gene expression of Tre metabolism genes in seeds. Regulation of gene expression will be monitored in a set of unique transgenic pea seed models with defined changes of their metabolic state. Regulation of promoter activities from these genes will be analysed in response to nutrient/hormone treatments. In addition, we want to unravel the regulation of class II Tre-P synthases by searching for protein interacting partners. Finally, we are planning a functional analysis of Tre metabolism during pea seed development by transgenic inhibition of gene expression. Levels of Tre-6-P will be modified by ectopic expression of E. coli Tre-P synthase and Tre-P phosphatase. Transgenic seeds will be subjected to phenotypic analysis by monitoring growth parameters and seed composition. From this project, we expect a more profound insight into the regulation of sugar metabolism and storage product synthesis of pea seeds in particular and of crop seeds in general.

种子新陈代谢必须与内源发育和外源环境因素相协调。这被认为是通过由SnRK1、脱落酸和海藻糖(Tre)途径介导的糖信号来实现的。Tre和Tre-6-P是植物中的次糖，但它们是整合和协调新陈代谢与发育的重要信号。在这个项目中，我们计划对豌豆种子发育过程中编码TrE代谢酶的基因进行分子和功能鉴定。具体地说，我们想要研究种子中TRE代谢基因的组织和细胞特异性基因表达。基因表达的调节将在一组独特的转基因豌豆种子模型中进行监测，这些模型具有明确的代谢状态变化。将分析这些基因对启动子活性的调节，以响应营养/激素处理。此外，我们希望通过寻找蛋白质相互作用伙伴来解开II类Tre-P合成酶的调节。最后，我们计划通过转基因抑制基因表达来分析豌豆种子发育过程中TrE代谢的功能。Tre-6-P的水平将通过大肠杆菌Tre-P合成酶和Tre-P磷酸酶的异位表达而改变。转基因种子将通过监测生长参数和种子成分进行表型分析。通过这个项目，我们期望对豌豆种子和农作物种子糖代谢和贮藏产物合成的调控有更深刻的了解。