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Wavelength dependence of UV-MALDI mass spectrometry and of direct UV-LDI-MS without matrix

UV-MALDI 质谱和无基质的直接 UV-LDI-MS 的波长依赖性

基本信息

批准号：
182585213
负责人：
Professor Dr. Klaus Dreisewerd
金额：
--
依托单位：
Universitäres Cancer Center Hamburg (UCCH)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2010
资助国家：
德国
起止时间：
2009-12-31 至 2016-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/182585213?language=en
关键词：
Wavelength dependence UV MALDI mass

项目摘要

In MALDI mass spectrometry, analytical sensitivity is largely influenced by the wavelength of the excitation laser. In the 1st funding period of the research project, the UV-MALDI desorption and ionization mechanisms were for the first time investigated systematically in the wavelength range of 280-377 nm by utilizing a selected set of matrix systems and peptides as analytes. These results showed that analytical performance can be substantially enhanced by adjusting the laser wavelength to the individual peak absorption of matrices. The 2nd funding period will build on these results in several important aspects. Established MALDI matrices and the analyte classes for which they are utilized will be comprehensively characterized with respect to the potential for improvement. Optimized matrix-wavelength systems that offer improved analytical sensitivity will be applied to spatially-resolved tissue imaging MS (MALDI-MSI). Next to standard matrices, liquid matrix systems that allow the generation of highly charged peptide ions will be characterized. Extension of the wavelength range to 213-450 nm furthermore allows to explore novel analytical possibilities, among which are, for example, the analysis of small molecules by matrix-free UV-LDI-MS and the UV-LDI-MSI analysis of metabolites in tissue. The studies will also deepen our knowledge about the basic MALDI mechanisms.

在MALDI质谱中，分析灵敏度很大程度上受激发激光波长的影响。在该研究项目的第一个资助期内，通过利用一组选定的基质系统和肽作为分析物，首次在280-377 nm波长范围内系统地研究了UV-MALDI解吸和电离机制。这些结果表明，通过将激光波长调整到基质的各个峰值吸收，可以显著提高分析性能。第二个供资期将在几个重要方面以这些成果为基础。已建立的MALDI基质及其使用的分析物类别将在改进潜力方面进行全面表征。优化的矩阵波长系统，提供更高的分析灵敏度将被应用于空间分辨组织成像MS（MALDI-MSI）。除了标准基质外，还将对允许产生高电荷肽离子的液体基质系统进行表征。波长范围扩展到213-450 nm还允许探索新的分析可能性，其中包括例如通过无基质UV-LDI-MS分析小分子和组织中代谢物的UV-LDI-MSI分析。这些研究也将加深我们对MALDI基本机制的了解。