Microbial Inventory Research Across Diverse Aquatic LTERs

不同水生 LTER 的微生物清单研究

• 批准号: 0717390
• 负责人: Linda Amaral Zettler
• 金额: $ 84万
• 依托单位: Marine Biological Laboratory
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-15 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0717390&HistoricalAwards=false
• 关键词: Microbial; Inventory; Research; Across; Diverse

This project will establish a Microbial Biodiversity Survey and Inventory across all 13 of the major aquatic (marine and freshwater) Long Term Ecological Research (LTER) sites established by the NSF US LTER Program. The long-term objective of the study is to document and describe baseline diversity and relative abundance data for both common and rare members of microbial communities located in aquatic LTER sites and to relate this diversity to the underlying physical and chemical environment. The project employs a new, high-volume DNA sequencing technology based on the sequencing of small ribosomal RNA regions that are especially variable, which will allow for discovery of novel microbial diversity in the bacterial, archaeal and eukaryal domains of life. Biodiversity in general, and microbial biodiversity in particular is important because microorganisms help shape Earth's habitability by orchestrating the major carbon transformations, biogeochemical cycles, and input into atmospheric composition. Radical fluctuations in microbial populations caused by changes in the environment pose irreversible threats to our quality of life and the entire biosphere. The resulting data will directly benefit participating LTERs that actively communicate results to policy makers, natural resource managers, the broader scientific community and the general public. Podcasts are planned to be coordinated with the Schoolyard LTER program.

该项目将在NSF US LTER计划建立的所有13个主要水生（海洋和淡水）长期生态研究（LTER）地点建立微生物生物多样性调查和库存。这项研究的长期目标是记录和描述水生长距离接触试验地点微生物群落常见和稀有成员的基线多样性和相对丰度数据，并将这种多样性与基本的物理和化学环境联系起来。该项目采用了一种新的高容量DNA测序技术，其基础是对特别可变的小核糖体RNA区域进行测序，这将有助于发现细菌、古细菌和真核生物领域的新型微生物多样性。生物多样性，特别是微生物生物多样性是重要的，因为微生物通过协调主要的碳转化，生物化学循环和输入大气成分来帮助塑造地球的可居住性。由环境变化引起的微生物种群的急剧波动对我们的生活质量和整个生物圈构成了不可逆转的威胁。由此产生的数据将直接有利于参与的长期环境要求，这些长期环境要求积极向决策者、自然资源管理者、更广泛的科学界和公众传达结果。播客计划与校园LTER计划协调。