Microbial Inventory Research Across Diverse Aquatic LTERs

该项目将在美国国家科学基金会长期生态研究（LTER）计划建立的所有13个主要水生（海洋和淡水）长期生态研究（LTER）站点建立微生物生物多样性调查和清单。该研究的长期目标是记录和描述水生LTER站点中常见和稀有微生物群落成员的基线多样性和相对丰度数据，并将这种多样性与潜在的物理和化学环境联系起来。该项目采用了一种新的、高容量的DNA测序技术，该技术基于对特别可变的小核糖体RNA区域的测序，这将允许在细菌、古细菌和真核生物领域发现新的微生物多样性。总体而言，生物多样性，尤其是微生物的生物多样性非常重要，因为微生物通过协调主要的碳转化、生物地球化学循环和大气成分的输入，帮助塑造地球的可居住性。环境变化造成的微生物种群急剧波动对我们的生活质量和整个生物圈构成了不可逆转的威胁。由此产生的数据将直接使参与的lter受益，这些lter积极地将结果传达给决策者、自然资源管理者、更广泛的科学界和一般公众。播客计划与校园LTER项目相协调。

This project will establish a Microbial Biodiversity Survey and Inventory across all 13 of the major aquatic (marine and freshwater) Long Term Ecological Research (LTER) sites established by the NSF US LTER Program. The long-term objective of the study is to document and describe baseline diversity and relative abundance data for both common and rare members of microbial communities located in aquatic LTER sites and to relate this diversity to the underlying physical and chemical environment. The project employs a new, high-volume DNA sequencing technology based on the sequencing of small ribosomal RNA regions that are especially variable, which will allow for discovery of novel microbial diversity in the bacterial, archaeal and eukaryal domains of life. Biodiversity in general, and microbial biodiversity in particular is important because microorganisms help shape Earth's habitability by orchestrating the major carbon transformations, biogeochemical cycles, and input into atmospheric composition. Radical fluctuations in microbial populations caused by changes in the environment pose irreversible threats to our quality of life and the entire biosphere. The resulting data will directly benefit participating LTERs that actively communicate results to policy makers, natural resource managers, the broader scientific community and the general public. Podcasts are planned to be coordinated with the Schoolyard LTER program.