SGER: Can specific proteins be detected and quantified in soil using proteomics techniques?

SGER：可以使用蛋白质组学技术检测和量化土壤中的特定蛋白质吗？

• 批准号: 0738040
• 负责人: Timothy Mattes
• 金额: --
• 依托单位: University of Iowa
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-11-15 至 2009-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0738040&HistoricalAwards=false
• 关键词: SGER; specific; proteins; detected; quantified

SGER ProposalCBET 0738040Tim MattesUniversity of IowaCan specific proteins be detected and quantified in soil using proteomics techniques?The primary objective of this research is to develop and apply proteomics techniques to detect and quantify a specific protein?the alpha subunit (EtnC) of the enzyme alkene monooxygenase (AkMO) from a strain of the bacterium Nocardioides, which has the capability of degrading vinyl chloride (VC). The AkMO enzyme catalyzes the first step in aerobic biodegradation of VC, which is a toxic intermediate in the biodegradation of chlorinated solvent trichloroethene, a widely distributed contaminant is soils and ground water. The ability to detect AkMO (via the subunit EtnC) in complex soil systems would be useful as a biomarker to signal the presence of VC-assimilating bacteria in soils. This proposal fits the criteria for an SGER in that it is an innovative idea and satisfies the high risk-high reward criterion. PI is a young tenure-track assistant professor, who is well qualified to conduct the proposed work and has ?state-of-the-art? analytical instrumentation at his disposal (liquid chromatography coupled with tandem mass spectrometry[lc-ms-ms]) for measuring protein macromolecules. Soil and ground-water contamination by chlorinated solvents is a significant societal problem. This work has the potential of opening a new area of research using proteomics techniques for the development of biomarkers.

SGER ProposalCBET 0738040Tim Mats爱荷华州大学能否使用蛋白质组学技术检测和定量土壤中的特定蛋白质？本研究的主要目标是开发和应用蛋白质组学技术来检测和定量特定蛋白质--来自具有降解氯乙烯(VC)能力的诺卡氏菌的一株菌株的烯烃单加氧酶(AkMO)的阿尔法亚单位(EtnC)。AkMO酶催化Vc好氧生物降解的第一步，Vc是氯化溶剂三氯乙烯生物降解的有毒中间体，三氯乙烯是土壤和地下水中广泛分布的污染物。在复杂的土壤系统中检测AkMO(通过EtnC亚基)的能力将是有用的，作为土壤中存在Vc同化细菌的生物标记物。这一建议符合SGER的标准，因为它是一个创新的想法，并满足高风险-高回报标准。Pi是一名年轻的终身教职助理教授，他非常有资格领导这项拟议的工作，并拥有？最先进的？他可以使用分析仪器(液-质联用[LC-MS-MS])来测量蛋白质大分子。氯化溶剂对土壤和地下水的污染是一个重大的社会问题。这项工作有可能开辟一个新的研究领域，使用蛋白质组学技术来开发生物标记物。