CAREER: RNA Folding Dynamics by Single Molecule Temperature Jump Kinetics

职业：通过单分子温度跳跃动力学研究 RNA 折叠动力学

• 批准号: 0747285
• 负责人: Christine Chow
• 金额: --
• 依托单位: Wayne State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-15 至 2013-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0747285&HistoricalAwards=false
• 关键词: CAREER; RNA; Folding; Dynamics; Single

In this CAREER award, Prof. David Rueda of Wayne State University and his graduate and undergraduate research students will develop new technology based on the combination of single molecule fluorescence detection with laser induced temperature jump (T-jump) thermal denaturation to study the structural dynamics of RNA molecules. The goal of the research is to understand how functional and structural heterogeneity in single RNA molecules affect the structure-function relationship in biologically important RNAs, as well as to establish a set of physical and chemical rules based upon this knowledge to engineer RNAs with useful applications. In addition to his research activities, Prof. Rueda will work with high school teachers in the predominantly African-American Detroit School District, through the Wayne State University Department of Education and the Office of Minority Student Initiatives. The bulk of this effort will be focused on developing a summer research program for high school science teachers. This project aims at filling an important gap in the continuous education program of high school science teachers by providing them with first hand experiences in a modern laboratory. Integration of research and education will also be achieved by actively supporting graduate, undergraduate and high school student training in the research laboratory.

在本次CAREER奖中，州立大学的韦恩教授大卫鲁埃达（David Rueda）及其研究生和本科生研究生将开发基于单分子荧光检测与激光诱导温度跳跃（T-jump）热变性相结合的新技术，研究RNA分子的结构动力学。该研究的目标是了解单个RNA分子中的功能和结构异质性如何影响生物学上重要的RNA中的结构-功能关系，以及建立一套基于这些知识的物理和化学规则来设计具有有用应用的RNA。除了他的研究活动，Rueda教授将与高中教师在主要是非洲裔美国人底特律学区，通过韦恩州立大学教育部和少数民族学生倡议办公室。这项工作的大部分将集中在为高中科学教师制定一个夏季研究计划。 该项目旨在填补高中科学教师继续教育计划的一个重要空白，为他们提供在现代实验室的第一手经验。还将通过积极支持研究生、本科生和高中生在研究实验室的培训，实现研究与教育的一体化。