Collaborative Research: A Systems Biology Approach for Metabolically Engineering Escherichia coli for Producing Hydrogen via Fermentation

合作研究：通过代谢工程大肠杆菌发酵生产氢气的系统生物学方法

• 批准号: 0753664
• 负责人: Ranjan Srivastava
• 金额: $ 7.58万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0753664&HistoricalAwards=false
• 关键词: Collaborative; Research; Systems; Biology; Approach

CBET-0753664SrivastavaNon-harmful bacteria may be used to produce chemicals for humans and this includes fuels. Molecular hydrogen is an environmentally-clean fuel; hence, if hydrogen can be synthesized efficiently by bacteria, then we may reduce air pollution, reduce our dependence on foreign oil, and avoid greenhouse gases (the carbon dioxide formed by our process will be recycled to plant mass and used again to form hydrogen so there are no net emissions). This research will enable hydrogen to be produced as a clean form of energy from bacteria using fermentation of simple sugars. In fermentative production of hydrogen, glucose is converted into pyruvate which is converted to formate which is then converted to hydrogen. Hence, it is imperative to direct the carbon flux in the bacterial cell to hydrogen formation by preventing competing glucose reactions and those that convert pyruvate to acetate, lactate, and ethanol or that remove formate. To achieve this aim, a systems biology approach will be used to produce more hydrogen that includes (i) altering the metabolism of the bacteria by removing unwanted pathways, (ii) evolving the necessary enzymes using protein engineering, and (iii) modeling all the metabolic pathways of the bacterial cell so that even better bacteria may be generated. We have chosen Escherichia coli as our model system since (i) all 3985 non-lethal single mutations of strain E. coli BW25113 are available; (ii) we may combine mutations rapidly using our novel, successive, virus-based method; (iii) E. coli is the best-studied bacterium so its metabolism is well understood; and (iv) its genome is sequenced so DNA microarrays are available and metabolic flux analysis may be carried out at the genome-scale. Along with the research results, the three professors involved in this research will train undergraduate and graduate students, will make accessible, via the World-Wide Web, specific outcomes of the project, and will disseminate some of the research results to a diverse audience using podcasting.

CBET-0753664 Srivastava无害细菌可用于生产人类化学品，包括燃料。 分子氢是一种环境清洁的燃料;因此，如果氢可以由细菌有效地合成，那么我们可以减少空气污染，减少对外国石油的依赖，并避免温室气体（我们的过程中形成的二氧化碳将被回收到工厂质量并再次用于形成氢，因此没有净排放）。 这项研究将使氢气作为一种清洁的能源形式从细菌中产生，使用简单的糖发酵。 在氢气的发酵生产中，葡萄糖转化为丙酮酸，丙酮酸转化为甲酸，甲酸然后转化为氢气。 因此，必须通过阻止竞争性葡萄糖反应以及将丙酮酸盐转化为乙酸盐、乳酸盐和乙醇或除去甲酸盐的反应，将细菌细胞中的碳通量引导至氢形成。 为了实现这一目标，将使用系统生物学方法来产生更多的氢，包括（i）通过去除不需要的途径来改变细菌的代谢，（ii）使用蛋白质工程来进化必要的酶，以及（iii）对细菌细胞的所有代谢途径进行建模，以便可以产生更好的细菌。 我们选择大肠杆菌作为我们的模型系统，因为（i）菌株E的所有3985个非致死性单突变; coli BW 25113的方法;（ii）我们可以使用我们的新的、连续的、基于病毒的方法快速地联合收割机组合突变;（iii）E.大肠杆菌是最好的研究细菌，所以它的代谢是很好的理解;和（iv）它的基因组测序，所以DNA微阵列是可用的，代谢通量分析可以在基因组规模进行。 沿着研究成果，参与这项研究的三位教授将培训本科生和研究生，将通过万维网提供该项目的具体成果，并将使用播客向不同的受众传播一些研究成果。