Investigation of structure and function of bacterial porins with special emphasis on their role in physiology of Borrelia and related species

研究细菌孔蛋白的结构和功能，特别强调它们在伯氏疏螺旋体和相关物种生理学中的作用

• 批准号: 187662720
• 负责人: Professor Dr. Roland Benz
• 金额: --
• 依托单位: Department of Life Sciences and Chemistry
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/187662720?language=en
• 关键词: Investigation; structure; function; bacterial; porins

The cytoplasm of gram-negative bacteria is surrounded by two membranes. The outer membranes serve as molecular filters. The filter properties are either general or specific. Small hydrophilic molecules pass these membranes with high velocity through channels that are known as bacterial porins. The project will deal with the study of these porin channels with special emphasis on the study of outer membrane channels of Borrelia and related Spirochetes, which are not well characterized to date. Borrelia are the cause of different diseases transmitted by the bites of parasites. For example Borrelia burgdorferi is the etiologic agent of Lyme borreliosis and is spread by Ixodes tics. Old world tick-borne relapsing fever (TBRF), caused in humans by the bite of an infected soft body tick Ornithodorus moubata transmitting the pathogen Borrelia duttonii, is a common endemic disease in Central, East and South Africa. Other pathogenic Borrelia species such as B. hermsii and B. recurrentis are also transmitted by tics or are louse-born. Borrelia spirochetes are hostpropagated bacteria that cycle between warm-blooded and arthropod hosts and have therefore developed advanced strategies to sense and survive in these environments. Uptake of nutrients through the outer membrane of Borrelia in different hosts plays an important role in the lifestyle of these bacteria because they have an only limited metabolic capacity. This means that the transport of nutrients through porins is very for important for survival of Borrelia in their hosts. Outer membrane porins in different Borrelia species and other Spirochetes should be identified, isolated, purified and characterized by different biochemical and biophysical methods. Molecular biology will be used to identify and clone porin genes. Porin genes should be recombinantly expressed in Escherichia coli for further analysis of the porin channels in different biochemical and biophysical approaches.

革兰氏阴性菌的细胞质被两层膜包围。外膜充当分子过滤器。过滤器属性可以是常规的，也可以是特定的。小的亲水性分子以高速通过这些膜，通过称为细菌孔蛋白的通道。该项目将处理这些孔蛋白通道的研究，特别强调疏螺旋体和相关螺旋体的外膜通道的研究，这是迄今为止没有得到很好的表征。疏螺旋体是由寄生虫叮咬传播的不同疾病的原因。例如，伯氏疏螺旋体是莱姆病的病原体，并通过硬蜱传播。旧世界蜱传回归热（TBRF），由感染的软体蜱Ornithodorus moubata叮咬人类引起，传播病原体Borrelia duttonii，是中非，东非和南非的常见地方病。其他致病性疏螺旋体属物种如B。hermsii和B.复发性皮炎也是通过抽搐传播的，或者是虱子传播的。疏螺旋体螺旋体是在温血动物和节肢动物宿主之间循环的宿主繁殖细菌，因此已经开发出先进的策略来感知这些环境并在这些环境中生存。在不同宿主中通过疏螺旋体的外膜摄取营养物质在这些细菌的生活方式中起着重要作用，因为它们只有有限的代谢能力。这意味着营养物质通过孔蛋白的运输对于疏螺旋体在其宿主中的存活非常重要。应通过不同的生物化学和生物物理方法鉴定、分离、纯化和表征不同疏螺旋体属物种和其他螺旋体中的外膜孔蛋白。分子生物学将用于鉴定和克隆孔蛋白基因。孔蛋白基因应重组表达在大肠杆菌中，进一步分析孔蛋白通道在不同的生物化学和生物物理方法。