Impact of specific long-chain-acyl-CoA synthetases on plant development

尽管有各种各样的高通量分子技术和生物信息学的理解个别蛋白质的生物学作用仍然远远没有完成。拟议的项目旨在为参与脂肪酸代谢的未表征蛋白质提供知识。具体而言，某些长链酰基辅酶A合成酶（拉克）对植物脂质代谢的作用将进行评估。由某些拉克突变等位基因引起的脂质组成的变化似乎对植物形态和植物发育产生了重大影响。在这个项目中，我们将集中在拉克基因与迄今未知的生物学作用。通过突变体分析，我们将剖析个人拉克的脂质代谢的变化，最终影响植物发育的贡献。将分析突变表型的脂质组成的生化变化以及对细胞和组织水平结构的影响。总体目标将是建立一个模型，解释所观察到的形态表型的分子基础。

Despite a wide variety of high-throughput molecular techniques and bioinformatics the understanding of the biological role of individual proteins is still far from being complete. The proposed project aims on contributing knowledge to such uncharacterized proteins involved in the fatty acid metabolism. Specifically, the role of certain long-chain-acyl-CoA synthetases (LACS) on the plant lipid metabolism will be evaluated. Changes in lipid composition provoked by certain LACS mutant alleles seem to impact plant morphology and plant development substantially. In this project we will focus on LACS genes with so far uncharacterized biological roles. By means of mutant analyses we will dissect the contribution of individual LACS to changes in lipid metabolism finally affecting plant development. Mutant phenotypes will be analyzed with respect to biochemical changes of the lipid composition and to the impact on structure on cellular and tissue level. The overall goal will be the establishment of a model explaining the molecular basis for the observed morphological phenotypes.