MRI: Acquisition of a Massively Parallel Sequencing Instrument System

康奈尔大学获得了罗氏454基因组测序仪FLX的资助。这种新的DNA测序仪器将使创造性的研究具有突破性发现的潜力。454系统可实现大规模并行DNA序列检测和分析。该仪器将由康奈尔大学生命科学核心实验室中心运营，作为全校共享的研究资源，这将使这项新技术具有成本效益。该仪器将用于全基因组组装、目标区域测序、突变检测、基因分型以及基因表达和基因调控的全基因组测量。该仪器将成为研究的一个重要工具，研究领域包括：改进作物育种的遗传标记、能够在植物和动物中引起疾病的细菌和真菌病原体、杀虫剂和杀真菌剂抗性、新陈代谢和免疫反应的调节、植物生育力、开花和果实成熟的调节，该基金将把最先进的测序技术应用于大学的研究和教育项目。454仪器将是推广计划的一部分，促进研究活动融入高中，本科和研究生教育水平的科学教学。该仪器将扩大研究和研究培训的共享资源，鼓励研究人员和学生使用新技术，并促进新测序研究应用的开发。454仪器将在推进各种生命科学研究的知识和理解方面发挥至关重要的作用。

A grant has been awarded to Cornell University for a Roche 454 Genome Sequencer FLX. This new DNA sequencing instrument will enable creative research with potential for breakthrough discoveries. The 454 system enables massively parallel DNA sequence detection and analysis. The instrument will be operated by the Cornell University Life Sciences Core Laboratories Center as a university-wide shared research resource, which will enable cost effective access to this new technology. The instrument will be used for whole genome assembly, sequencing of targeted regions, mutation detection, genotyping, and genome-wide measurements of gene expression and gene regulation. The instrument will be an essential tool in research that includes studies on genetic markers for improved crop breeding, bacterial and fungal pathogens capable of causing disease in plants and animals, insecticide and fungicide resistance, the regulation of metabolism and of the immune response, the regulation of fertility, flowering and fruit ripening in plants, and genome level regulation of crop biomass.This grant will place state-of-the-art sequencing technology into university research and education programs. The 454 instrument will be part of outreach programs that facilitate the integration of research activities into the teaching of science at the high school, undergraduate and graduate educational levels. The instrument will expand shared resources for research and research training, stimulate use of a new technology by researchers and students, and foster the development of new sequencing research applications. The 454 instrument will play a crucial role in advancing knowledge and understanding in a wide variety of life science research.