International Collaboration in Chemistry: Mechanism of operation of the BLUF domain: blue light sensitive biosensors

国际化学合作：BLUF 域的运行机制：蓝光敏感生物传感器

• 批准号: 0822587
• 负责人: Peter Tonge
• 金额: $ 34.5万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0822587&HistoricalAwards=false
• 关键词: International; Collaboration; Chemistry; Mechanism; operation

The Analytical and Surface Chemistry Program at NSF Division of Chemistry will support the international collaborative research project of Prof. Peter Tonge of State University of New York at Stony Brook and Prof. Stephen Meech of the University of East Anglia. Profs. Tonge and Meech and their students will focus on AppA, a photoreceptor from the photosynthetic bacterium Rhodobacter sphaeroides which uses a Blue Light Using Flavin adenine dinucleotide (BLUF) domain to respond to light. Their hypothesis is that the primary event in the photoactivation of AppA is an alteration in the electronic structure of the flavin isoalloxazine ring in the excited state, which causes a rearrangement in hydrogen bonding interactions between protein and chromophore on the picosecond time scale. Ultrafast time resolved fluorescence (UTRF) will be used to reveal excited state dynamics, while femtosecond to millisecond time resolved vibrational spectroscopy (TRIR) will be used to observe the influence of photoexcitation on the host matrix structure. Data interpretation will utilize isotopic substitution and polarization measurements, coupled with the incorporation of unnatural amino acids. The resulting detailed structure- dynamics relationship will yield the first complete insight into the microscopic mechanism of photoreceptor operation. The study could lead to the development of a new generation of protein-based biosensors. The study will provide excellent training opportunities to US and UK students who will gain invaluable international research experience through extended research stays in the collaborators' laboratories. This international collaborative research project is supported jointly by NSF and the Engineering and Physical Sciences Research Council (EPSRC) in the United Kingdom. The study is also supported by the Office of International Science and Engineering (OISE) at NSF.

NSF化学部的分析和表面化学计划将支持纽约州立大学石溪分校的Peter Tonge教授和东英吉利大学的Stephen Meech教授的国际合作研究项目。教授们。Tonge和Meech以及他们的学生将专注于Appa，这是光合作用细菌球形红杆菌的一种光感受器，它使用黄素腺嘌呤二核苷酸(BLUF)结构域的蓝光来对光做出反应。他们的假设是，APPA光活化的主要事件是黄素异四氢嘧啶环在激发态的电子结构的改变，这导致蛋白质和发色团之间的氢键相互作用在皮秒尺度上的重排。超快时间分辨荧光(UTRF)将用于揭示激发态动力学，而飞秒到毫秒时间分辨振动光谱(TRIR)将用于观察光激发对基质结构的影响。数据解释将利用同位素替代和极化测量，再加上非天然氨基酸的加入。由此产生的详细的结构-动力学关系将首次完整地洞察光感受器工作的微观机制。这项研究可能会导致新一代基于蛋白质的生物传感器的开发。这项研究将为美国和英国的学生提供极好的培训机会，他们将通过延长在合作者实验室的研究时间获得宝贵的国际研究经验。这一国际合作研究项目由NSF和英国工程和物理科学研究理事会(EPSRC)联合支持。这项研究也得到了美国国家科学基金会国际科学与工程办公室(OISE)的支持。