Patterning the Micromeres in the Spiralian Blastula

螺旋囊胚中微粒的图案化

• 批准号: 0844734
• 负责人: J. David Lambert
• 金额: $ 51万
• 依托单位: University of Rochester
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0844734&HistoricalAwards=false
• 关键词: Patterning; Micromeres; Spiralian; Blastula

During the development of multicellular organisms, proper cell fates can be specified by asymmetric cell divisions, where key molecules are distributed unequally between two sister cells. Despite the importance of this mechanism for normal development, it remains poorly understood. The embryo of the mollusc Ilyanassa has numerous asymmetric cell divisions and important practical and experimental advantages, making it a useful model for understanding this process. This group of researchers has discovered that many RNA molecules in the embryo are specifically localized and segregated during early development by a novel mechanism. This project will empirically determine the sequences that control localization and asymmetric segregation of these RNAs, allowing comparison of the RNA structures that mediate similar and different patterns of localization. The identification of these RNA sequences will then allow these researchers to test hypotheses about how the specificity of localization is achieved. These experiments will also test whether the localization of one of these RNAs is important for cell fate specification by using functional gene-knockdown approaches. These experiments should significantly expand understanding of how RNAs are sorted during cell division, and how this impacts early embryogenesis. The work will also train young scientists at the graduate and undergraduate levels for future careers as independent researchers and scholars in this underexplored area of cell differentiation.

在多细胞生物的发育过程中，细胞的命运可以通过不对称的细胞分裂来确定，其中关键分子在两个姐妹细胞之间不均匀地分布。 尽管这种机制对正常发育很重要，但人们对它的了解仍然很少。 软体动物Ilyanassa的胚胎具有许多不对称的细胞分裂和重要的实践和实验优势，使其成为了解这一过程的有用模型。 这组研究人员发现，胚胎中的许多RNA分子在早期发育过程中通过一种新的机制被特异性定位和分离。 该项目将凭经验确定控制这些RNA的定位和不对称分离的序列，从而比较介导相似和不同定位模式的RNA结构。这些RNA序列的鉴定将使这些研究人员能够测试关于如何实现定位特异性的假设。 这些实验还将通过使用功能性基因敲低方法来测试这些RNA之一的定位对于细胞命运规范是否重要。 这些实验应该大大扩展了对RNA在细胞分裂过程中如何分选以及这如何影响早期胚胎发生的理解。 这项工作还将培养研究生和本科生层次的年轻科学家，为未来的职业生涯作为独立的研究人员和学者在这一探索不足的细胞分化领域。