IRES: U.S.-Czech Research Experience for Students: Structure and Function of Novel Flavoprotein (WrbA)

这个学生国际研究经验奖（IRES）将普林斯顿大学的Jannette Carey与捷克南波希米亚大学物理生物学研究所的同行联系起来。在捷克共和国，IRES项目将由Ivana Smatanova和Rudiger Ettrich领导，并与该大学的蛋白质结构和功能系合作。这些美国和捷克导师的共同目标是促进我们对大肠杆菌黄蛋白WrbA的生化特性的理解，同时通过对新型WrbA和相关蛋白质功能的指导研究，为他们指导的本科生提供早期职业研究经验。在IRES合作的过程中，美捷团队的研究计划将通过提供野生型和突变菌株生长研究的第一手研究经验、代谢谱、酶动力学、x射线晶体学和分子模型来吸引学生参与者。如果成功，研究人员和学生的共同努力有望更好地定义WrbA的机制和生理作用，从而使结构-功能关系的详细图景得以展现。这个跨学科的IRES生物分子科学项目实现了推进科学知识和吸引学生的项目目标，使美国和欧洲的专家能够在共同感兴趣和能力强的领域结合互补的人才和共享研究资源。更广泛的影响包括在职业生涯早期向美国本科生介绍国际专业知识和团队合作，使他们更好地为全球劳动力做好准备。通过研究相对简单的大肠杆菌，美国和捷克合作的成果可能有助于解释其他生物体的膜解毒机制。如果成功，对WrbA酶的了解可能会影响生物化学的其他研究，特别是与有趣的可溶性脱氢酶的研究。

OISE 0853423CareyThis award for International Research Experience for Students (IRES) links Jannette Carey from Princeton University with Czech counterparts from the Institute of Physical Biology at the University of South Bohemia, in Nove Hrady. In the Czech Republic, the IRES program will be headed by Ivana Smatanova and Rudiger Ettrich, with the University's Department of Structure and Function of Proteins. These U.S. and Czech mentors share the goal of contributing to our understanding of the biochemical properties of the E. coli flavoprotein WrbA while providing the undergraduate students they mentor with early career research experience through directed study of the function of the novel WrbA and related proteins. During the course of the collaborative IRES, the US-Czech team's research plans will engage student participants by providing first hand research experience with growth studies of wild-type and mutant strains, metabolic profiling, enzyme kinetics, x-ray crystallography, and molecular modeling. If successful, the combined efforts of researchers and students are expected to better define the WrbA mechanism and physiological roles, allowing for a detailed picture of structure-function relationships to emerge. This interdisciplinary IRES program in biomolecular science fulfills the program objective of advancing scientific knowledge and engaging students by enabling experts in the United States and Europe to combine complementary talents and share research resources in areas of strong mutual interest and competence. Broader impacts include early career introduction of U.S. undergraduate students to international expertise and teamwork to better prepare them for the global workforce. By working with the relatively simple organism, E. coli, the results of this U.S.-Czech effort may help explain how membranes detoxify in other organisms. If successful, knowledge of the enzyme WrbA may influence other research in biochemistry, particularly work with the interesting soluble diaphorase enzymes.