Complexin and synaptotagmin in Ca2+-triggered exocytosis (A11)

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The project investigates the action of CpxII and its interplay with the major Ca2+ sensors Synaptotagmin I and VII in Ca2+-triggered exocytosis of chromaffin cells. Starting from the corresponding null mutants (single and double null mutants) and in the framework of detailed structure-function analysis, we aim to achieve this goal by using a combination of electrophysiological, biochemical, and fluorescence microscopic techniques (confocal/STED). Furthermore, we will study CpxII’s role in gliotransmission from astrocytes and will investigate its impact on integrative functions of hippocampal signaling.