Complexin and synaptotagmin in Ca2+-triggered exocytosis (A11)

Ca2 触发的胞吐作用中的复合蛋白和突触结合蛋白 (A11)

• 批准号: 192422421
• 金额: --
• 依托单位: Center for Integrative Physiology and Molecular Medicine (CIPMM)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/192422421?language=en
• 关键词: Complexin; synaptotagmin; Ca2+; triggered; exocytosis

The project investigates the action of CpxII and its interplay with the major Ca2+ sensors Synaptotagmin I and VII in Ca2+-triggered exocytosis of chromaffin cells. Starting from the corresponding null mutants (single and double null mutants) and in the framework of detailed structure-function analysis, we aim to achieve this goal by using a combination of electrophysiological, biochemical, and fluorescence microscopic techniques (confocal/STED). Furthermore, we will study CpxII’s role in gliotransmission from astrocytes and will investigate its impact on integrative functions of hippocampal signaling.

该项目研究了CpxII的作用及其与主要Ca2+传感器Synaptotagmin I和VII在Ca2+触发的染色质细胞胞外分泌中的相互作用。从相应的零突变体（单零突变体和双零突变体）开始，在详细的结构-功能分析框架内，我们的目标是通过结合电生理、生化和荧光显微技术（共聚焦/STED）来实现这一目标。此外，我们将研究CpxII在星形胶质细胞胶质传递中的作用，并研究其对海马信号综合功能的影响。