Substrate recognition by the HRD-ubiquitin ligase (B05)

HRD-泛素连接酶 (B05) 的底物识别

• 批准号: 192594405
• 金额: --
• 依托单位: Max-Delbrück-Centrum für Molekulare Medizin (MDC) in der Helmholtz-Gemeinschaft
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/192594405?language=en
• 关键词: Substrate; recognition; HRD; ubiquitin; ligase

One of the best-studied ubiquitin ligases involved in protein quality control is the HMG-CoA reductase degradation-(HRD-) ligase. It is embedded in the endoplasmic reticulum (ER) membrane and constitutes the central component of a process termed ER-Associated Protein Degradation (ERAD). We want to investigate the interaction of the main components of this Module with misfolded client proteins and with the folding machinery of the ER. Besides biochemical in vitro studies and yeast genetics, this includes cryoEM and a CLMS approach.

参与蛋白质质量控制的研究最多的泛素连接酶之一是HMG-CoA还原酶降解-（HRD-）连接酶。它包埋在内质网（ER）膜中，构成称为ER相关蛋白降解（ERAD）过程的中心组分。我们希望研究该模块的主要组分与错误折叠的客户蛋白和ER折叠机制的相互作用。除了生物化学体外研究和酵母遗传学，这包括cryoEM和CLMS方法。