Postdoctoral Research Fellowships in Biology for FY 2009

2009财年生物学博士后研究奖学金

• 批准号: 0905958
• 负责人: Benjamin Blackman
• 金额: --
• 依托单位: Blackman Benjamin K
• 依托单位国家: 美国
• 项目类别: Fellowship
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0905958&HistoricalAwards=false
• 关键词: Postdoctoral; Research; Fellowships; Biology; FY

This action funds an NSF Postdoctoral Research Fellowship for FY 2009. The fellowship supports a research and training plan entitled "Environmental contributions to gene expression evolution during local adaptation and gene family expansion" for Benjamin Blackman. The host institution for this research is Duke University and the sponsoring scientist is John Willis.Evolutionary changes in gene regulation often play important roles in how natural populations adapt to their local habitats and also how different copies of the same gene persist after duplication. This research examines how variation in gene expression contributes to habitat adaptation and gene family diversification in the yellow monkeyflower, Mimulus guttatus. Next-generation sequencing technology is being used to assess variation in gene expression observed in Mimulus grown across a variety of habitats: coastal areas with high soil salinity, inland dry habitats, and hybrid plants, reared both in greenhouses and in the wild. Analyzing expression on a genomic scale in multiple environmental conditions provides insight into three questions regarding how the environment and gene expression evolution interact. First, how are mutations affecting environmental regulation of gene expression distributed in the genome relative to the position of the gene they regulate? Second, how are ancestral and evolved environmental responses in gene expression related? Finally, to what extent do gene families diverge with respect to expression in different environments?Training objectives include learning laboratory, computational, and programming skills necessary for collecting, analyzing, and distributing genome-scale datasets. Broader impacts include an important database (an initial expression atlas for Mimulus) and new bioinformatics tools for analyzing gene expression patterns. This research also advances knowledge about the molecular basis of two important agronomic traits, responses to drought and salt stress.

这项行动为2009财政年度的NSF博士后研究奖学金提供资金。该研究金支持Benjamin Blackman的一项研究和培训计划，题为“在当地适应和基因家族扩展期间环境对基因表达进化的贡献”。这项研究的主办机构是杜克大学，赞助科学家是约翰·威利斯。基因调控的进化变化通常在自然种群如何适应其当地栖息地以及相同基因的不同拷贝在复制后如何持续存在方面起着重要作用。本研究探讨了如何在基因表达的变化有助于栖息地适应和基因家族的多样化，在黄色monkeyflower，Mimulus guttatus。下一代测序技术正被用于评估在多种栖息地生长的Mimulus中观察到的基因表达变化：高土壤盐度的沿海地区，内陆干燥的栖息地，以及在温室和野外饲养的杂交植物。在多种环境条件下分析基因组规模上的表达提供了关于环境和基因表达进化如何相互作用的三个问题的见解。首先，影响基因表达的环境调控的突变在基因组中的分布与它们所调控的基因的位置有关吗？第二，基因表达中的祖先和进化环境反应是如何相关的？最后，基因家族在不同环境中的表达差异有多大？培训目标包括学习收集，分析和分发基因组规模数据集所需的实验室，计算和编程技能。更广泛的影响包括一个重要的数据库（Mimulus的初始表达图谱）和用于分析基因表达模式的新生物信息学工具。这项研究还推进了对干旱和盐胁迫反应这两个重要农艺性状的分子基础的认识。