Functional organization and dynamics of PI-kinase-based modules for protein sorting at endosomal membranes (C08)

内体在营养素、受体和细胞信号分子的摄取、再循环和降解中起作用。在此之前，我们的特点是磷脂酰肌醇（PI）4-激酶II型为基础的蛋白质模块在核内体差异调节Wnt信号。在即将到来的资助期内，我们将剖析PI 3 KC 2 α与Raptor和HOPS以及PI 4KIIa/β与外囊之间复合物形成的分子基础。我们将分析这些PI激酶模块如何在时间和空间上组织，它们如何有助于内溶体膜稳态，以及它们如何调节细胞信号传导。

Endosomes function in the uptake, recycling, and degradation of nutrients, receptors, and cell signaling molecules. Previously, we have characterized phosphatidylinositol (PI) 4-kinase type II-based protein modules at endosomes that differentially regulate Wnt signaling. During the forthcoming funding period we will dissect the molecular basis underlying complex formation between PI3KC2ß with Raptor and HOPS and of PI4KIIa/ß and exocyst. We will analyze how these PI kinase modules are organized in time and space, how they contribute to endolysosomal membrane homeostasis, and how they regulate cell signaling.