BEST: A novel molecular approach to measuring In situ feeding rates of copepods in the South Eastern Bering Sea.

最佳：一种测量东南白令海桡足类原位摄食率的新颖分子方法。

• 批准号: 0909415
• 负责人: Edward Durbin
• 金额: $ 57.42万
• 依托单位: University of Rhode Island
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0909415&HistoricalAwards=false
• 关键词: BEST; novel; molecular; approach; measuring

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Durbin 0909415University of Rhode islandMesozooplankton play a critical role in determining carbon flow from primary producers to higher trophic levels. In the SE Bering Sea interannual changes in the timing and extent of both sea ice cover and summer stratification will affect their role in determining the path of carbon flow, whether through the pelagic ecosystem or to the benthos. Knowledge of the actual in situ mesozooplankton diet composition and ingestion rates is essential to understanding the flow of carbon in this system. Information about these rates using traditional bottle incubation techniques will be compromised because of the strong vertical gradients in the mesozooplankton prey environment making sampling the appropriate prey field difficult. The PIs propose a field investigation of in situ feeding rates by copepods on the SE Bering shelf during spring and summer using a novel molecular technique they have developed to quantitatively measure ingestion rates on different prey species from stomach content analysis. They will use the 18S ribosomal RNA gene as a marker to identify different prey species present in the guts of copepods and quantitative real-time polymerase chain reaction (q-PCR) to measure copy number of this gene for each prey species. Ingestion rates will be calculated from this gut content information, 18S copy number/organism, and DNA digestion rates. By combining these in situ ingestion rate measurements with zooplankton abundance measurements and the prey field composition, they will be able to determine the fate of phytoplankton production under the varying environmental conditions. Ultimately, they plan to use these in situ consumption estimates to evaluate the role of copepods in carbon flow in the Bering shelf ecosystem.

该奖项是根据2009年的《美国复苏与再现法案》（公法111-5）进行资助的。Durbin0909415 Rhode Islandmesozookooplankton的University在确定从主要生产国到较高营养水平的碳流动方面起着关键作用。在Se Bering海洋中，海冰覆盖和夏季分层的时间和程度的年度变化将影响其在确定碳流动路径的作用，无论是通过骨膜生态系统还是到底栖生物。了解实际原位中龙龙饮食组成和摄入率对于理解该系统中的碳的流动至关重要。使用传统的瓶孵化技术的信息将被损害，因为中龙普兰克顿猎物环境中的垂直梯度很强，这使得对适当的猎物场进行了采样。 PI提出了在春季和夏季，使用一种新型的分子技术对春季贝林架上的copepods对原位喂养率进行现场调查，以从胃含量分析中定量测量不同猎物物种的摄入率。他们将使用18S核糖体RNA基因作为标志物，以识别copepods肠道中存在的不同猎物物种和定量的实时聚合酶链反应（Q-PCR）来测量每个猎物物种的拷贝数。摄入率将从此肠道内容信息，18S拷贝数/生物和DNA消化率中计算出来。通过将这些原位摄入速率测量与浮游动物的丰度测量和猎物场组成相结合，它们将能够在不同的环境条件下确定浮游植物产量的命运。最终，他们计划使用这些原位消费估计来评估CopePods在白令货架生态系统中碳流中的作用。