RUI: Testing phylogenetic hypotheses within Tubulinea, a clade of free-living lobose amoebae

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Much remains to be learned about the biological diversity of microbial organisms. Amoebae, the subject of this proposal, have been particularly neglected because of both their small size and simple cell structures that provide few characters for comparative studies. This project relies on molecular tools to elucidate the diversity of amoebae in the group Tubulinea, with a focus on the so-called shelled amoebae in the Arcellinida. These amoebae, which are abundant in diverse habitats including ponds, streams, and many soils, are likely important players in food webs and in Carbon cycling. Project deliverables include: deposition of new cultures of free-living amoebae, characterization of numerous genes from diverse amoebae; and creation of a framework for future studies of these poorly known species.The broader impacts of this project include: 1) training undergraduates and graduate students; 2) educational outreach to local schools, including those in the underserved city of Springfield, Massachusetts; and 3) increased participation of students from underrepresented groups, including first generation college students, through a program co-founded by the principal investigator at Smith College.