RUI: The cell biology of pectin dynamics in the Charophycean Green Algae: Homogalacturonan secretion in the model organism, Penium margaritaceum

RUI：轮藻类绿藻中果胶动力学的细胞生物学：模型生物 Penium margaritaceum 中的同半乳糖醛酸分泌

• 批准号: 0919925
• 负责人: David Domozych
• 金额: $ 23.2万
• 依托单位: Skidmore College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2012-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0919925&HistoricalAwards=false
• 关键词: RUI; cell; biology; pectin; dynamics

Intellectual Merit Cell wall polymers are important biochemicals in the growth, development and survival of green plants and constitute the most abundant biochemicals on the planet. Pectins represent one of the major classes of cell wall polymers and function in regulating cell wall expansion and cell growth as well as cell wall porosity and uptake of key ions. The synthesis, secretion and post-secretory remodeling of pectins represent key subcellular events in the life of a plant cell and specific studies to elucidate these processes have only just begun. Pectins are found in all land plant groups and have been recently characterized in the Charophycean Green Algae (CGA). The CGA represent the extant group of green algae that is ancestral to modern-day land plants. In this project, a model organism from the CGA, Penium margaritaceum, is employed to study quantitative aspects of pectin secretion, the specific role of subcellular components in pectin and cell wall processing, and the role of a key pectin-modifying enzyme. This project applies various experimental, microscopy-based and immunolabeling technologies to studies of the endomembrane system, cytoskeletal/cytomotile networks, and cell surface phenomena that are intricately involved in the secretion of pectins to the cell wall. The results of this project will be synthesized and used in the construction of models of pectin dynamics in plants and will provide insight into cell wall polymer processing by green plant cells, the coordinated interaction of multiple subcellular systems in defined secretory activities, and the evolution of primitive green plants. Broader Impacts This RUI project will provide summer research opportunities for 4 undergraduates and semester-based research opportunities for 6-10 undergraduates at Skidmore College. The project also will promote the integration of research in education as components of the project will be used to enhance instruction and support laboratory experiences in courses as Plant Biology, Plant Physiology, and Biological Electron Microscopy. The project also includes outreach experiences for pre-college students. Specifically the outreach plan includes a series of active presentations by the PI and Skidmore biology majors to elementary students and their teachers on the microscopic world. In addition the PI and his students will lead Saturday field trips to wetlands for high school and middle school students and their teachers. The field trips will include activities in collecting microscopic specimen followed by identification exercises with the microscope. The results of the study will be broadly disseminated through student/PI presentations and publications, outreach programs, and the recently established CGA website at Skidmore College.

智力优点 细胞壁聚合物是绿色植物生长、发育和生存的重要生化物质，是地球上最丰富的生化物质。果胶代表细胞壁聚合物的主要类别之一，具有调节细胞壁扩张和细胞生长以及细胞壁孔隙度和关键离子吸收的功能。果胶的合成、分泌和分泌后重塑代表了植物细胞生命中的关键亚细胞事件，阐明这些过程的具体研究才刚刚开始。 果胶存在于所有陆地植物群中，最近在轮藻类绿藻 (CGA) 中得到了表征。 CGA 代表现存的绿藻类群，是现代陆地植物的祖先。 在该项目中，来自 CGA 的模型生物 Penium margaritaceum 用于研究果胶分泌的定量方面、亚细胞成分在果胶和细胞壁加工中的具体作用以及关键果胶修饰酶的作用。该项目应用各种基于显微镜的实验和免疫标记技术来研究内膜系统、细胞骨架/细胞运动网络以及与果胶分泌到细胞壁密切相关的细胞表面现象。 该项目的结果将被合成并用于构建植物果胶动力学模型，并将深入了解绿色植物细胞的细胞壁聚合物加工、多个亚细胞系统在确定的分泌活动中的协调相互作用以及原始绿色植物的进化。 更广泛的影响 该 RUI 项目将为斯基德莫尔学院的 4 名本科生提供夏季研究机会，并为 6-10 名本科生提供学期研究机会。该项目还将促进研究与教育的整合，因为该项目的组成部分将用于加强植物生物学、植物生理学和生物电子显微镜等课程的教学和支持实验室经验。 该项目还包括针对大学预科学生的外展体验。 具体来说，外展计划包括 PI 和斯基德莫尔生物学专业的学生向小学生及其老师进行一系列关于微观世界的积极演讲。此外，首席研究员和他的学生将在周六带领高中生和初中生及其老师前往湿地进行实地考察。 实地考察将包括收集显微标本的活动，然后进行显微镜识别练习。 研究结果将通过学生/PI 演示和出版物、外展计划以及斯基德莫尔学院最近建立的 CGA 网站进行广泛传播。