RUI: A proteomic analysis of stress responses in the ribbed salt marsh mussel, Geukensia demissa

RUI：肋盐沼贻贝 Geukensia demissa 应激反应的蛋白质组学分析

• 批准号: 0920103
• 负责人: Peter Fields
• 金额: $ 20.92万
• 依托单位: Franklin and Marshall College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-15 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0920103&HistoricalAwards=false
• 关键词: RUI; proteomic; analysis; stress; responses

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).The salt marsh mussel, Geukensia demissa, is an important member of the salt marsh community that stretches from northern Florida to the Gulf of Saint Lawrence. In this habitat, Geukensia helps stabilize sediment, filters particulates from the water, and provides habitat for many species of invertebrates. Because it lives in the intertidal zone, Geukensia must cope with environmental extremes including high and low temperatures, anoxia (absence of oxygen), desiccation and fluctuations in salinity. Students in the Fields lab will determine the biochemical strategies Geukensia employs to survive in this challenging habitat. The research will employ proteomics (an analysis of changes in types and abundances of proteins in tissue, including identification of proteins whose abundances change significantly) to examine how Geukensia responds to environmental stresses. The research will address the following questions: How much change in an environmental variable is necessary to modify protein expression (a stress response)? Are the same groups of proteins expressed in response to different stresses? Can proteins whose expression changes significantly be identified? Answers to these questions will provide insight into how Geukensia is able to survive in such a variable environment. More generally, the research will reveal similarities in biochemical responses to a variety of stresses, and the extent to which tolerance to one type of stress provides resistance to others. Answers to these questions have important ramifications regarding the ability of organisms to survive in the face of human-induced stresses, including climate change. In addition to addressing the above questions, this research will engage undergraduates in research, exposing them to biochemistry techniques including 2-D gel electrophoresis and mass spectrometry. Undergraduates will also have the opportunity to co-author peer-reviewed articles and present their results at national meetings.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。盐沼贻贝，Geukensia demissa，是盐沼社区的重要成员，从北方佛罗里达延伸到圣劳伦斯湾。在这个栖息地，Geukensia有助于稳定沉积物，过滤水中的颗粒物，并为许多无脊椎动物提供栖息地。由于它生活在潮间带，Geukensia必须科普极端环境，包括高温和低温，缺氧（缺氧），干燥和盐度波动。菲尔兹实验室的学生将确定Geukensia在这个具有挑战性的栖息地中生存的生化策略。该研究将采用蛋白质组学（分析组织中蛋白质的类型和丰度的变化，包括鉴定丰度发生显著变化的蛋白质）来研究Geukensia如何应对环境压力。该研究将解决以下问题：环境变量的变化需要多少才能改变蛋白质表达（应激反应）？相同的蛋白质组在不同的压力下表达吗？表达发生显著变化的蛋白质能否被识别？这些问题的答案将提供深入了解Geukensia如何能够在这样一个多变的环境中生存。更一般地说，这项研究将揭示对各种压力的生化反应的相似性，以及对一种压力的耐受性在多大程度上为其他压力提供了抵抗力。这些问题的答案对于生物体在人类引起的压力，包括气候变化中的生存能力具有重要影响。除了解决上述问题，这项研究将从事研究的本科生，暴露他们的生物化学技术，包括2-D凝胶电泳和质谱。本科生也将有机会共同撰写同行评审的文章，并在全国会议上展示他们的成果。