RIG: Role of Cdx transcription factors in the control of neural plate competence to FGF pattering signals

RIG：Cdx 转录因子在控制神经板 FGF 模式信号能力中的作用

• 批准号: 0920449
• 负责人: Isaac Skromne
• 金额: $ 20万
• 依托单位: University of Miami
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0920449&HistoricalAwards=false
• 关键词: RIG; Role; Cdx; transcription; factors

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).A neural progenitor cell encounters numerous cues during its development, but acquires a distinct fate from hundreds of possible fates. The PI is interested in understanding how neural progenitor cells choose the cues to respond to during their development. For example, his previous work demonstrated that a cell's decision to become brain or spinal cord was controlled by Caudal/Cdx genes. This project examines the role the Fibroblast Growth Factors (FGFs) signaling in regulating Caudal/Cdx activity during the cell?s decision. He will address this issue by altering Caudal/Cdx and FGF gene expression at different times and places during zebra fish development. He expects to uncover the conditions where FGF signaling promotes proliferation and differentiation of brain and spinal cord progenitor cells and where these processes are prevented from occurring. These studies should provide new insights into the mechanisms governing the ability of cells to respond to environmental factors during the development and adult life of an organism. In particular, this research should have broad intellectual implications in the fields of immunology, neurobiology, developmental and cancer biology. This research program is also designed to foster inquiry-based research and communication skills and advance knowledge among graduate and undergraduate students, particularly those from underrepresented minorities.

该奖项是根据2009年美国复苏和再投资法案(公法111-5)资助的。神经前体细胞在其发展过程中遇到许多线索，但从数百种可能的命运中获得了截然不同的命运。PI感兴趣的是了解神经前体细胞在其发育过程中如何选择要响应的线索。例如，他之前的工作证明，细胞成为大脑或脊髓的决定是由Caudal/CDX基因控制的。本项目研究成纤维细胞生长因子(FGFs)信号在细胞-S决定过程中调节尾部/CDX活性中的作用。他将通过改变斑马鱼发育过程中不同时间和地点的Caudal/CDX和FGF基因表达来解决这个问题。他希望发现成纤维细胞生长因子信号促进脑和脊髓前体细胞增殖和分化的条件，以及这些过程在哪里被阻止发生。这些研究应该为控制细胞在有机体发育和成体生命过程中对环境因素做出反应的能力提供新的见解。特别是，这项研究应该在免疫学、神经生物学、发育生物学和癌症生物学领域具有广泛的智力影响。这项研究计划还旨在培养基于探究的研究和沟通技能，并在研究生和本科生中增进知识，特别是那些来自代表性不足的少数族裔的学生。

项目成果

Retinoic acid regulates size, pattern and alignment of tissues at the head-trunk transition

• DOI: 10.1242/dev.109603
• 发表时间: 2014-11-01
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Lee, Keun;Skromne, Isaac
• 通讯作者: Skromne, Isaac