Role of the presynaptic protein Mover at auditory brain stem synapses (B02)

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Mover is a recently identified component of the presynaptic machinery. We plan to i) determine the role of Mover at excitatory and inhibitory synapses in acute slices of the cochlear nucleus, ii) characterize its contribution to homeostatic plasticity in hearing impaired mice, and iii) determine the underlying molecular mechanisms by analyzing how the expression and localization of Mover are regulated in the brain and in cultured neurons. Our primary tool will be a floxed Mover mouse line that we created in the first period. Using Cre lines we will delete Mover in distinct subsets of neurons, to study synaptic transmission by patch clamping and synaptic architecture by light and electron microscopy.