Synaptic vesicle priming in murine rod bipolar cell ribbon synapses (B01)

鼠杆双极细胞带状突触中的突触小泡启动 (B01)

• 批准号: 193726128
• 金额: --
• 依托单位: Abteilung Molekulare Neurobiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/193726128?language=en
• 关键词: Synaptic; vesicle; priming; murine; rod

Retinal ribbon synapses exhibit 5-10 times higher priming rates than conventional synapses, but the molecular basis of this unique feature is unknown. We propose to study synaptic vesicle priming in mouse rod bipolar cell to AII amacrine cell ribbon synapses, focusing our efforts on mice lacking Munc13s and CAPSs. We will analyze synaptic transmission at these synapses in wild-type and KO mutant mice, and will complement these studies with analyses of the ultrastructure of rod bipolar cell synapses in wild-type and KO mutant mice. We expect that our studies will define the role of synaptic vesicle priming in determining the unique properties of retinal ribbon synapses.

视网膜带状突触表现出比传统突触高5-10倍的启动率，但这种独特特征的分子基础尚不清楚。我们拟研究小鼠杆状双极细胞到AII无长突细胞带状突触的突触囊泡启动，重点是缺乏Munc 13和CAPS的小鼠。我们将分析野生型和KO突变小鼠的这些突触的突触传递，并将补充这些研究与野生型和KO突变小鼠的视杆双极细胞突触的超微结构分析。我们希望我们的研究将确定的作用，突触囊泡启动在确定视网膜带状突触的独特属性。