MRI-RUI: Acquisition of a Spinning confocal Microscope to Advance Interdepartmental Research and Training at Missouri State University-Springfield

MRI-RUI：购买旋转共焦显微镜以推进密苏里州立大学斯普林菲尔德分校的跨部门研究和培训

• 批准号: 0923024
• 负责人: Kyoungtae Kim
• 金额: $ 24.84万
• 依托单位: Missouri State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923024&HistoricalAwards=false
• 关键词: MRI; RUI; Acquisition; Spinning; confocal

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).A grant has been awarded to Missouri State University (MSU) to acquire a laser spinning confocal fluorescence microscope equipped with a Yokogawa spinning confocal scanner and four laser lines for research and teaching at the university and in the region. This instrument will not only allow researchers to achieve significantly improved quality in microscopic imaging but will also afford them the opportunity to perform advanced techniques such as fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET), optical sectioning of specimens, and dual-channel real-time imaging. All participants including the PI and Co-PIs represent an interdepartmental group of faculty who, despite diverse research interests, possess commonalities in their employed approaches and instrumentation. Foreseeable outcomes from the research activities include advancement of interdisciplinary research, training, and collaboration. This new system will offer researchers the exciting opportunity to pursue several up to date research avenues including: 1) uncovering the roles of dynamin-like proteins in endocytosis, 2) characterizing anti-inflammatory signaling networks downstream of interleukin-10, 3) revealing the role of ATAF1 in plant resistance to powdery mildew pathogens, 4) understanding the role of actin cytoskeleton reorganization during the defense responses by grapevine to powdery mildew pathogens, 5) monitoring the delivery of carbon nanotubes in cells, 6) monitoring fluorescent biosensors based on conducting polymer nanowires, 7) studying neuron-glial cell communication, 8) investigating fecal coliform growth in biofilms derived from freshwater streams, and 9) studying P2 nucleotide receptor function at the molecular level. Employment of the confocal microscopy in multiple classroom settings at the university and in the region will broaden the impact of the instrument. Training with the instrument will be incorporated into the curriculums for five of our academic departments, both in traditional classes and student independent research. Acquisition of the spinning confocal system will impact many facets of the MSU community and beyond. An immediate outcome will be the expanded investigative capacity of the major users and students. As a result, faculty in several departments will be better positioned to (i) contribute to the scientific community by way of conference presentations and peer-reviewed publications and (ii) compete for external research funding. A heightened research profile at MSU will aid in the recruitment and retention of high-quality faculty and students. Furthermore, the establishment of the proposed system will broaden interdisciplinary collaboration with local universities. Collectively, greater success in these areas will increase student research opportunities and heighten the quality of undergraduate and graduate education in the region. The availability of this instrument will also contribute greatly towards the outreach programs in the local community to educate families and motivate young people to pursue careers in science and engineering. Outcomes of the research projects will be communicated through presentations by students and faculty at regional or national meetings, in peer reviewed journal publications, and on the internet.

该奖项是根据2009年的《美国回收与再投资法》（公法111-5）资助的。已向密苏里州立大学（MSU）授予了赠款，以获取配备有横川旋转共焦扫描仪和四个激光线的激光旋转共聚焦荧光显微镜，用于该地区和该地区的研究和教学。该仪器不仅将使研究人员能够在微观成像中显着提高质量，而且还可以使他们有机会执行高级技术，例如光漂白后的荧光恢复（FRAP）（FRAP），荧光共振能量传递（FRET），样品的光学分段，以及双通道实时成像。包括PI和Co-Pis在内的所有参与者都代表了一个部门间的教职员工，尽管研究兴趣多样化，但在其使用的方法和仪器中都有共同点。研究活动的可预见结果包括跨学科研究，培训和协作的进步。这个新系统将为研究人员提供一个令人兴奋的机会，以追求几种最新的研究途径，包括：1）揭示类似动力蛋白的蛋白质在内吞作用中的作用，2）表征抗炎信号网络interleukin-10，3），3）在植物上的作用中，ATCERT在植物上的作用，了解ATCERT的作用，理解Atter的作用，理解ActiN cyer的作用，4） grapevine to powdery mildew pathogens, 5) monitoring the delivery of carbon nanotubes in cells, 6) monitoring fluorescent biosensors based on conducting polymer nanowires, 7) studying neuron-glial cell communication, 8) investigating fecal coliform growth in biofilms derived from freshwater streams, and 9) studying P2 nucleotide receptor function at the molecular level.在大学和该地区的多个教室环境中使用共聚焦显微镜将扩大乐器的影响。该乐器的培训将在我们五个学术部门的传统班级和学生独立研究中纳入课程中。 获取旋转共聚焦系统将影响MSU社区及其他地区的许多方面。直接的结果将是主要用户和学生的调查能力的扩大。结果，多个部门的教师将通过会议演讲和同行评审的出版物和（ii）竞争外部研究资金来更好地为科学界做出更好的定位。密西根州立大学的研究概况的提高将有助于招募和保留高质量的教职员工和学生。此外，拟议系统的建立将扩大与当地大学的跨学科合作。总体而言，在这些领域取得更大的成功将增加学生的研究机会，并提高该地区的本科和研究生教育的质量。该工具的可用性还将为当地社区的外展计划做出巨大贡献，以教育家庭并激励年轻人从事科学和工程学的职业。 研究项目的成果将通过学生和教职员工在区域或国家会议，同行评审的期刊出版物以及互联网上进行传达。