MRI: Acquisition of an Inverted Confocal Microscope for Florida Gulf Coast University

0923030Geiger“该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。”佛罗里达海湾海岸大学（FGCU）的研究人员正在向国家科学基金会申请资金，以购买一台倒置共聚焦显微镜，并将其安置在最近成立的（2005年）美国大学惠特克工程学院（WSOE）的新家霍姆斯大厅。该显微镜将由奥林巴斯FluoView 1000共聚焦系统组成，围绕倒置奥林巴斯IX81显微镜配置。之所以选择奥林巴斯系统，是因为它的零漂移技术和激光强度反馈系统将为延长持续时间的实验提供卓越的延时成像，这对于粒子跟踪和确定细胞内粒子的命运至关重要。此外，IX81将配置为独立的标准荧光显微镜，包括反褶积显微镜。总的来说，这台显微镜将为现有设备提供重大改进，并将使FGCU和邻近机构的研究人员有可能扩大他们的研究范围，包括细胞和亚细胞成像。该系统的主要使用将围绕三个主要调查人员，一个（盖格）在WSOE，两个（伊瑟恩和迈克尔）在艺术与科学学院（CAS）。这三人都隶属于FGCU的惠特克科学、技术、工程和数学（STEM）教育中心，包括担任临时联合主任（Ise.0rn）和该中心的本科生研究导师（全部）。中国科学院对其理工科本科生有研究需求，在建立博士后培养计划的帮助下，中国科学院的研究人员不仅培养了本科生研究人员，而且在一流期刊上发表了他们的研究成果，并将他们的本科生研究人员安置在一流的研究生项目、医学院、生物科学领域的高薪职位和K-12科学教育中。

0923030Geiger"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."Investigators at Florida Gulf Coast University (FGCU) are requesting funding from the National Science Foundation to purchase an inverted confocal microscope to be housed in Holmes Hall, the new home of the recently-formed (2005) U.A. Whitaker School of Engineering (WSOE). This microscope will consist of an Olympus FluoView 1000 confocal system configured around an inverted Olympus IX81 microscope. The Olympus system was selected because its zero-drift technology and laser intensity feedback system will allow for superior time-lapse imaging for extended duration experiments that are crucial for particle tracking and determining intracellular particle fate. In addition, the IX81 will be configured for independent standard epifluorescence microscopy, including deconvolution microscopy. Overall, this microscope will provide a significant improvement to the facilities currently available and will make it possible for researchers at FGCU and neighboring institutions to expand their research to include cellular and sub-cellular imaging. The main use of this system will revolve around three primary investigators, one (Geiger) within the WSOE and two (Isern and Michael) within the College of Arts and Sciences (CAS). All three are affiliated with FGCU's Whitaker Center for Science, Technology, Engineering and Mathematics (STEM) Education at various levels, including serving as interim co-director (Ise.0rn) and acting as undergraduate research mentors within the center (all). The CAS has a research requirement for their science undergraduates, and the CAS investigators with the aid of an established postdoctoral training program have a proven track record for not only training undergraduate researchers, but also for publishing their research in leading journals and for placing their undergraduate researchers in top-tier graduate programs, medical schools, high-wage positions in the bioscience sector, and K-12 science education.