Impact of altered flooding and crop rotation regimes on microbial communities and activities involved in nitrogen fixation and nitrogen cycling

改变洪水和作物轮作制度对微生物群落以及固氮和氮循环活动的影响

• 批准号: 194674098
• 负责人: Professorin Dr. Barbara Reinhold-Hurek
• 金额: --
• 依托单位: Arbeitsgruppe Microbe-Plant Interactions
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/194674098?language=en
• 关键词: Impact; altered; flooding; crop; rotation

We will study the effect of crop rotation between upland maize and submerged rice cultivation and between flooded and non-flooded conditions on the processes involved in methane production and on the composition of the methanogenic microbial community. This will be done by a combination of δ13C analysis in root organic carbon, soil organic carbon, acetate, CO2 and CH4; of pulse-labelling experiments using 13C-CO2; and of molecular analysis of the 16S rRNA gene of the methanogenic archaeal community in soil and rhizosphere. As result we expect to obtain turnover rates of carbon, the relative contribution of different pathways to CH4 production and the relative contribution of root-derived versus soil organic matter-derived carbon to CH4 production, all these parameters in relation to the composition of the methanogenic archaeal community. The study will show how crop rotation will affect community composition and processes involved in production and emission of the green-house gas methane.

我们将研究旱地玉米与水稻田、淹水与非淹水轮作对甲烷生产过程和产甲烷微生物群落组成的影响。通过综合分析根系有机碳、土壤有机碳、乙酸、CO2和CH4的δ13C；13C-CO2脉冲标记实验；土壤和根际产甲烷古菌群落16S rRNA基因的分子分析。因此，我们期望得到碳的周转率、不同途径对CH4产生的相对贡献以及根源碳和土壤有机质碳对CH4产生的相对贡献，所有这些参数都与产甲烷古菌群落的组成有关。这项研究将展示作物轮作将如何影响群落组成以及温室气体甲烷的生产和排放过程。