Isothermal Calorimetry in Physical Chemistry and Biochemistry Laboratories

化学(12)正在为使用等温滴定量热法(ITC)的高级物理化学和生物化学实验室课程开发实验室实验。这些练习包括：“EDTA与钙(II)和镁(II)离子的结合常数”；“胰酶对苯并精氨酸乙酯(BAEE)的米氏酶动力学研究”；“N-乙酰氨基葡萄糖(NAG)3配基与溶菌酶的结合”；以及“钙调素与钙离子/钙调素激酶II的相互作用的研究”。两种试剂的结合亲和力、反应热和化学计量比直接从ITC实验中获得。此外，通过组合这些反应参数，还可以确定所研究反应的反应熵和Gibb自由能的变化。项目评估将包括这些创新教材对学生学习的影响。等温滴定量热法是一种相对较新的技术，用于确定小规模反应的热力学参数。等温量热法的跨学科性质使其成为展示发现和药物开发等社会效益之间联系的有力工具。现代的、超灵敏的ITC在生物化学和药学研究中有许多应用：蛋白质-蛋白质、蛋白质-药物、蛋白质-DNA、蛋白质-配体和金属-配体相互作用是可以研究的分子间相互作用的例子。这些努力产生的教育材料正在全国化学教育会议上传播，并通过向专业期刊提交的手稿进行传播。

Chemistry (12) Laboratory experiments are being developed for upper-level Physical Chemistry and Biochemistry Laboratory courses that utilize isothermal titration calorimetry (ITC). The exercises include: "Binding Constants of EDTA with Ca(II) and Mg(II) Ions"; "Michaelis-Menten Enzyme Kinetic Study of the Hydrolysis of Benzoylarginine Ether Ester (BAEE) by Trypsin"; "Binding of the N-Acetylglucosamine Trisaccharide ((NAG)3)Ligand to Lysozyme"; and "Investigation of the Interaction of Calmodulin with Ca2+/Calmodulin Kinase II". The binding affinity, reaction enthalpy, and stoichiometric ratio for the two reagents are being obtained directly from the ITC experiments. Additionally, by combining these reaction parameters, it is also possible to determine the reaction entropy and the change in Gibb's free energy for the reaction studied. Program assessment will include the impact of these innovative educational materials on student learning.Isothermal titration calorimetry is a relatively new technique that is used to determine thermodynamic parameters for small scale reactions. The interdisciplinary nature of isothermal calorimetry makes it a powerful tool to demonstrate the link between discovery and societal benefits such as drug development. Modern, ultrasensitive ITC has found many applications in Biochemistry and Pharmaceutical research: protein-protein, protein-drug, protein-DNA, protein-ligand and metal-ligand interactions are examples of intermolecular interactions that can be studied. Educational materials resulting from these efforts are being nationally disseminated at chemistry education conferences and via manuscripts submitted to professional journals.