Isothermal Calorimetry in Physical Chemistry and Biochemistry Laboratories

化学（12）利用等温滴定量热法（ITC）的高级物理化学和生物化学实验课程正在开发实验室实验。练习包括：“EDTA与Ca（II）和Mg（II）离子的结合常数”；Michaelis-Menten酶水解苯甲酰精氨酸醚酯（BAEE）的动力学研究n -乙酰氨基葡萄糖三糖（(NAG)3）配体与溶菌酶的结合钙调蛋白与Ca2+/钙调蛋白激酶II相互作用的研究。这两种试剂的结合亲和力、反应焓和化学计量比直接从ITC实验中得到。此外，通过结合这些反应参数，还可以确定所研究反应的反应熵和吉布自由能的变化。项目评估将包括这些创新教育材料对学生学习的影响。等温滴定量热法是一种用于测定小尺度反应热力学参数的新技术。等温量热法的跨学科性质使其成为展示发现与药物开发等社会效益之间联系的有力工具。现代，超灵敏的ITC在生物化学和制药研究中有许多应用：蛋白质-蛋白质，蛋白质-药物，蛋白质- dna，蛋白质-配体和金属-配体相互作用是可以研究的分子间相互作用的例子。这些努力所产生的教育材料正在全国化学教育会议上传播，并通过向专业期刊提交手稿的方式传播。

Chemistry (12) Laboratory experiments are being developed for upper-level Physical Chemistry and Biochemistry Laboratory courses that utilize isothermal titration calorimetry (ITC). The exercises include: "Binding Constants of EDTA with Ca(II) and Mg(II) Ions"; "Michaelis-Menten Enzyme Kinetic Study of the Hydrolysis of Benzoylarginine Ether Ester (BAEE) by Trypsin"; "Binding of the N-Acetylglucosamine Trisaccharide ((NAG)3)Ligand to Lysozyme"; and "Investigation of the Interaction of Calmodulin with Ca2+/Calmodulin Kinase II". The binding affinity, reaction enthalpy, and stoichiometric ratio for the two reagents are being obtained directly from the ITC experiments. Additionally, by combining these reaction parameters, it is also possible to determine the reaction entropy and the change in Gibb's free energy for the reaction studied. Program assessment will include the impact of these innovative educational materials on student learning.Isothermal titration calorimetry is a relatively new technique that is used to determine thermodynamic parameters for small scale reactions. The interdisciplinary nature of isothermal calorimetry makes it a powerful tool to demonstrate the link between discovery and societal benefits such as drug development. Modern, ultrasensitive ITC has found many applications in Biochemistry and Pharmaceutical research: protein-protein, protein-drug, protein-DNA, protein-ligand and metal-ligand interactions are examples of intermolecular interactions that can be studied. Educational materials resulting from these efforts are being nationally disseminated at chemistry education conferences and via manuscripts submitted to professional journals.