Genetic and Molecular Characterization of Two Quantitative Trait Loci for Circadian Rhythm in Neurospora crassa

粗糙脉孢菌昼夜节律的两个数量性状基因座的遗传和分子特征

• 批准号: 0946860
• 负责人: Kwangwon Lee
• 金额: $ 62.5万
• 依托单位: Rutgers University Camden
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0946860&HistoricalAwards=false
• 关键词: Genetic; Molecular; Characterization; Two; Quantitative

Most eukaryotic organisms display cellular and behavioral rhythms with a period of approximately 24 hours. These rhythms are collectively called the biological or circadian "clock", and this clock influences many of an organism's activities, such as the sleep/wake cycle in humans. Through genetic, biochemical, molecular, and genomic studies, the filamentous fungus Neurospora crassa has served as an excellent model system for unraveling many biological mysteries. Due to the commonality of the core mechanisms of the circadian clock in eukaryotes, understanding the clock in Neurospora should improve our understanding of its functioning and biological consequences in a wide range of organisms. The goal of this project is the molecular characterization of two major quantitative trait loci (QTLs) that contribute to circadian rhythms. The project should provide insights into the biology of both the circadian clock and quantitative trait loci. This research will be integrated into a new laboratory module for the investigator's "Circadian Rhythms" course. The laboratory module will provide undergraduate students - science majors and non-majors alike - with an opportunity for hands-on experience in the experimental characterization of the fungal circadian clock, along with insights into the nature and study of quantitative traits. This will be an invaluable learning experience for these students, and will help them develop into the intellectually informed, scientifically sophisticated citizens that the nation needs.

大多数真核生物显示细胞和行为节律，周期约为24小时。 这些节律统称为生物钟或昼夜节律“时钟”，这种时钟影响生物体的许多活动，例如人类的睡眠/觉醒周期。 通过遗传学、生物化学、分子生物学和基因组学研究，丝状真菌粗糙脉孢菌已成为解开许多生物学奥秘的极好模型系统。由于真核生物中生物钟核心机制的共同性，了解脉孢菌中的生物钟应该有助于我们了解其在各种生物体中的功能和生物学后果。 该项目的目标是两个主要的数量性状基因座（QTL），有助于昼夜节律的分子特征。 该项目应提供生物钟和数量性状基因座的生物学见解。这项研究将被整合到一个新的实验室模块的研究人员的“昼夜节律”课程。 实验室模块将为本科生-科学专业和非专业的学生一样-在真菌生物钟的实验表征的动手经验的机会，沿着洞察到的性质和数量性状的研究。 这将是一个宝贵的学习经验，为这些学生，并将帮助他们发展成为知识渊博，科学成熟的公民，国家需要。