Development of a Synchrotron Beamline for High-resolution Microdiffraction Analysis of Biological Macromolecules

开发用于生物大分子高分辨率微衍射分析的同步加速器光束线

• 批准号: 0960034
• 负责人: Wayne Hendrickson
• 金额: $ 350万
• 依托单位: New York Structural Biology Center Incorporated
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0960034&HistoricalAwards=false
• 关键词: Development; Synchrotron; Beamline; resolution; Microdiffraction

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the development of a synchrotron beamline for high-resolution microdiffraction analysis of biological macromolecules at the New York Structural Biology Center (NYSBC). The NYSBC is a consortium of ten New York institutions that provides infrastructure to support structural biology. NYSBC scientists are developing the synchrotron beamline on a new undulator source (X5) at the National Synchrotron Light Source (NSLS) to support current needs of the NYSBC community and to anticipate opportunities that new undulators at NSLS-II will provide. Microdiffraction is the defining capability of the new beamline, and this is a property in high demand by NYSBC scientists and other biological crystallographers. The new beamline provides highly focused x-ray beams for use in crystallographic analyses of a wide range of biological macromolecules, including enzymes, membrane proteins, DNA, RNA and assemblages of multiple macromolecular components, and is based on a novel monochromator design that focuses the undulator radiation into stable microbeams with exceptional energy resolution for optimization of anomalous scattering phasing experiments. NYSBC-affiliated laboratories train hundreds of graduate students and postdoctoral trainees, and many of them will benefit from using the new beamline in their research. The broader impacts include the training of students and fellows who will use this equipment and the societal impacts of basic discoveries made from experiments at the new beamline. In addition, innovations made in this project benefit industries associated with this development. Results from the studies enabled by the new equipment are disseminated by student and faculty presentations at regional and national meetings, and through publication in peer-reviewed journals.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。这项主要研究仪器-回收和再投资（MRI-R2）奖资助了纽约结构生物学中心（NYSBC）用于生物大分子高分辨率微衍射分析的同步加速器光束线的开发。NYSBC是一个由十个纽约机构组成的联盟，为结构生物学提供基础设施支持。NYSBC科学家正在国家同步加速器光源（NSLS）的新波动源（X5）上开发同步加速器光束线，以支持NYSBC社区当前的需求，并预测NSLS- ii的新波动将提供的机会。微衍射是新光束线的定义能力，这是NYSBC科学家和其他生物晶体学家高度要求的特性。新的光束线提供高度聚焦的x射线光束，用于广泛的生物大分子的晶体学分析，包括酶，膜蛋白，DNA， RNA和多种大分子组分的组合，并且基于一种新的单色器设计，将波动辐射聚焦到稳定的微光束中，具有卓越的能量分辨率，用于优化异常散射相位实验。nysbc附属实验室培养了数百名研究生和博士后学员，他们中的许多人将受益于在他们的研究中使用新的光束线。更广泛的影响包括培训将使用该设备的学生和研究员，以及在新光束线进行的实验中取得的基本发现的社会影响。此外，在这个项目中进行的创新使与此开发相关的行业受益。由新设备促成的研究成果通过学生和教师在区域和国家会议上的发言以及在同行评议的期刊上发表的方式传播。